水稻独脚金内酯信号感知的激活和终止
- 1湖南大学生物学院, 化学生物传感与计量学国家重点实验室, 植物功能基因组学与发育调控湖南省重点实验室, 长沙 410082
2清华大学生命科学学院, 生物信息学教育部重点实验室, 清华-北大生命科学联合中心, 北京 100084
姚瑞枫, 湖南大学教授, 博士生导师, 教育部“青年长江学者”, 国家重点研发“青年首席”。主要从事植物激素作用机理和生物育种研究, 在新型激素独脚金内酯的感知和信号转导机制方面做出了一定的学术贡献, 相关成果以通讯作者或第一作者发表在Nature、Nat Comm、Plant Cell、Mol Plant和Cell Res等期刊。其中“植物分枝激素独脚金内酯的受体感知机制”曾入选中国生命科学十大进展、中国高等学校十大科技进展。兼任中国蛋白质组学专委会委员、中国植物学会植物整合组学专委会委员, 作为秘书长参与组织了第3届国际独脚金内酯大会。E-mail: ryao@hnu.edu.cn
收稿日期: 2024-10-28
录用日期: 2024-11-02
网络出版日期: 2024-11-04
基金资助
国家重点研发计划青年科学家项目(2022YFF1002000)
Activation and Termination of Strigolactone Signal Perception in Rice
- 1Hunan Provincial Key Laboratory of Plant Functional Genomics and Developmental Regulation, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha 410082, China
2MOE Key Laboratory of Bioinformatics, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China
Received date: 2024-10-28
Accepted date: 2024-11-02
Online published: 2024-11-04
摘要
独脚金内酯(strigolactone, SL)是调控植物分枝等重要生长发育过程的新型植物激素。水稻(Oryza sativa)中SL受体D14感知SL信号, 结合F-box蛋白D3并招募转录抑制子D53, 诱导D53泛素化降解, 从而触发信号传递、抑制分蘖。最近的一项研究发现, 低氮信号诱导D14的N端无序区(NTD)特异位点发生磷酸化修饰, 造成D14的泛素化降解减弱, 从而增强SL信号通路。此外, 低氮信号本身可以诱导SL合成, 通过这两种机制的协同作用强化SL信号转导, 从而强烈抑制分蘖, 使水稻适应低氮逆境。该研究还发现, SL诱导的D14-D3相互作用也促进了D14的泛素化降解, 从而介导SL信号感知的终止。这些重要发现阐明了水稻中SL信号感知的激活和终止机制, 揭示了SL信号在控制水稻分蘖以适应低氮逆境中的重要作用, 为理解植物如何适应营养匮乏等外界环境变化提供了新的重要见解, 对作物株型的精准改良以及减肥增产水稻分子设计育种具有重要指导意义。
本文引用格式
姚瑞枫 , 谢道昕 . 水稻独脚金内酯信号感知的激活和终止[J]. 植物学报, 2024 , 59(6) : 873 -877 . DOI: 10.11983/CBB24163
Abstract
Strigolactone (SL) is a novel plant hormone that regulates important growth and developmental processes such as plant branching. In rice, the SL receptor D14 perceives SL signals, binds with the F-box protein D3, and recruits the transcriptional repressor D53, inducing the ubiquitination and degradation of D53, thereby triggering signal transduction and inhibiting tillering. A recent study discovered that nitrogen limitation induces SL biosynthesis in rice to activate the receptor D14, triggering SL signal transduction. Concurrently, nitrogen limitation also induces phosphorylation of the N-terminal disordered region (NTD) of D14, reducing the ubiquitination and degradation of receptor D14, thereby further enhancing SL perception. Through these two synergistic mechanisms, nitrogen limitation stimulates SL signal transduction, strongly inhibiting tillering and enabling rice to adapt to low nitrogen stress conditions. The study also found that the D14-D3 interaction induced by SL promotes the ubiquitination and degradation of D14, thereby mediating the termination of SL signal perception. These significant findings elucidate the mechanisms of activation and termination of SL perception in rice, revealing the crucial regulatory role of SL signals in controlling rice tillering under low nitrogen stress. This would provide key insights into plant adaptation to nutrient scarcity and guide the precise improvement of crop architecture and molecular breeding of rice for reduced fertilizer use and increased yield.
Key words： rice; strigolactone; receptor D14; nitrogen limitation; phosphorylation; ubiquitination
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