植物学报 ›› 2002, Vol. 19 ›› Issue (02): 231-233.

• 研究简报 • 上一篇    下一篇

剥粒菠萝组织培养及快速繁殖的研究

罗丽霞 程萍   

  1. (珠海市农业科学研究中心 珠海 519070)
  • 收稿日期:2001-09-20 修回日期:2001-12-18 出版日期:2002-03-20 发布日期:2002-03-20
  • 通讯作者: 程萍

Study on Tissue Culture and Macropropagation of Peeling-pineapple

Li-Xia CHENG Ping   

  1. (Zhuhai Agricultural Science Research Center, Zhuhai 519070)
  • Received:2001-09-20 Revised:2001-12-18 Online:2002-03-20 Published:2002-03-20

摘要: 剥粒菠萝是目前菠萝品种中最适于鲜食的良种之一。本研究以剥粒菠萝为材料,通过10种培养基的诱导筛选、繁殖生根,从中选出最适宜剥粒菠萝组培快繁的一整套稳定、高效的生产程序,即:芽诱导培养基为MS+6_BA 3 mg/L(单位下同)+NAA 0.1,增殖培养基为MS+6-BA4+NAA 0.1,生根培养基为1/2MS+6-BA 2+NAA 0.2+活性炭0.1%。该组培技术的成功,为规模化生产剥粒菠萝提供了快速繁殖种苗的技术保障,有利于促进南方地区名优水果的发展,有利于南方农业结构的产业化调整。

Abstract: Peeling_pineapple (Anana comosus Merr. `Baoli') is one of the impr oved varieties of pineapples which can be eaten uncooked. The stem tips of peeling-pineapple seedlings were used as explants. After screening experiments from 10 culture media for bud induction and rooting, a stable high efficient procedure for macropropagation of peeling_pineapple was established. The proper bud induction medium was MS+6_BA 3mg/L (the unit is the same below)+NAA 0.1; the mult iplication medium was MS+6_BA 4+NAA 0.1; the rooting medium was 1/2MS+6_BA 2+NAA 0.2+active carbon 0.1%. We offer a macropropagation method to the large scale production of peeling_pineapple plants.