Abstract: Zinc finger proteins play crucial roles in regulating plant growth and development, and response to stresses in plants. To study the roles of zinc finger proteins in response to abiotic stresses, we cloned rice OsBBX25, encoding a novel zinc finger protein with a B-box domain. The expression of OsBBX25 was induced by salt, drought and abscisic acid. Overexpression of OsBBX25 in Arabidopsis thaliana resulted in increased tolerance to salt and drought stress. The expression of KIN1, RD29A and COR15 was upregulated in response to salt stress, and that of KIN1, RD29A and RD22 was promoted in response to drought stress in transgenic plants. With exogenous application of ABA, the seed germination rates of the wild-type and transgenic plants were similar. OsBBX25 may act as a co-transcriptional activator to regulate the expression of stress-related genes to respond to abiotic stress.