Abstract: We studied in vitro culture regeneration from cotyledons of 11 genotypes of radish. Those genotypes, which have a high rate of regeneration, were used as the test materials for subsequent experiments of explant type, hormone ratio, and seedling age. The regenerated plantlets, which generated roots well, were transplanted into the field. The entire cotyledon with a petiole is the optimal regeneration explant. Four days of seedling age is suitable to induce adventitious shoots. The maximum frequency of shoot regeneration was 86.95% obtained from cotyledons of Z3 in the medium Murishige and Skoog + 6 mg·L^–1 6-BA + 0.05 mg·L^–1 NAA, and the regeneration coefficient reached 1.80. These results set the basis for radish genetic transformation.