Abstract:

Embryogenic callus was initiated from bamboo (Dendrocalamus latiflorus Munro) anthers cultured on M₈ medium supplemented with 2 mg·L^–1 NAA, 0.5 mg·L^–1 6-BA, 15 mg·L^–1 phenylacetic acid (PAA), 7.5 mg·L^–1 silver thiosulfate (STS), 500 mg·L^–1 casein enzymatic hydrolysate (CH), 100 mg·L^–1 proline, 100 mg·L^–1 glutamine, 5.4% maltose, and 0.8% agar. Subculture of these embryogenic calli on the same medium resulted in embryoid formation and their subsequent germination to form rooted plantlets. A one-step method for anther culture and plant regeneration of D. latiflorus was preliminarily established.