Chin Bull Bot ›› 2018, Vol. 53 ›› Issue (2): 203-211.doi: 10.11983/CBB17026

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

New Evidence for the Mode of Extracellular Freezing in Leaves of Ligustrum lucidum and Euonymus japonicus under Low Temperatures

Ruifeng Yu, Jianjun Zhu*   

  1. College of Life Sciences, Ludong University, Yantai 264025, China
  • Received:2017-02-18 Revised:2017-05-04 Accepted:2017-05-09 Online:2017-06-06 Published:2018-03-01
  • Contact: Jianjun Zhu E-mail:jzjzhu@gmail.com

Abstract: The freezing mode, namely intracellular or extracellular freezing, is vital to plants under low temperatures, but direct evidence of extracellular freezing, especially from thermodynamic experiments, are still very rare. In this paper, we examined exotherms from differential thermal analyses as well as microscopic observations in detached leaves from Ligustrum lucidum and Euonymus japonicus during cooling. We show new evidence of extracellular freezing during cooling. The living leaves of the two plants consistently exhibited biphasic freezing exotherms (i.e., an initial small exotherm followed by a slow but much larger exotherm). In contrast, the freeze-killed leaves, as well as the filter paper drenched with extracted tissue solutions from leaves of the two plants, exhibited just one fast and large exotherm typical for a single-phase freezing. Extensive colourless extracellular ice crystals were seen under the microscope, with cells dehydrated and shrunken but with no leakage of coloured cell solution, which agreed with the thermal analysis. The experiments presented a new approach and deeper insight into the mechanisms of freezing stress and injury in higher plants.

No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Zhang Zhen-jue. Some Principles Governing Shedding of Flowers and Fruits in Vanilla fragrans[J]. Chin Bull Bot, 1985, 3(05): 36 -37 .
[2] Qian Gao;Yuying Liu;Yinan Fei;Dapeng Li;Xianglin Liu* . Research Advances into the Root Radial Patterning Gene SHORT-ROOT[J]. Chin Bull Bot, 2008, 25(03): 363 -372 .
[3] Wang Bao-shan;Zou Qi and Zhao Ke-fu. Advances in Mechanism of Crop Salt Tolerance and Strategies for Raising Crop Salt Tolerance[J]. Chin Bull Bot, 1997, 14(增刊): 25 -30 .
[4] HE Feng WU Zhen-Bin. Application of Aquatic Plants in Sewage Treatment and Water Quality Improvement[J]. Chin Bull Bot, 2003, 20(06): 641 -647 .
[5] TIAN Bao-Lin WANG Shi-Jun LI Cheng-Sen CHEN Gui-Ren. An Approach on the Origin Center, Evolution Center and the Mechanics of Evolution and Extinction of the Late Palaeozoic Cathaysian Flora[J]. Chin Bull Bot, 2000, 17(专辑): 21 -33 .
[6] ZHANG Yan FANG Li LI Tian-Fei YAO Zhao-BingJIANG Jin-Hui. Effect of Calcium on the Heat Tolerance and Active Oxygen Metabolism of Tobacco Leaves[J]. Chin Bull Bot, 2002, 19(06): 721 -726 .
[7] JIA Hu-Sen LI De-QuanHAN Ya-Qin. Cytochrome b-559 in Chloroplasts[J]. Chin Bull Bot, 2001, 18(02): 158 -162 .
[8] Wei Sun;Chonghui Li;Liangsheng Wang;Silan Dai*. Analysis of Anthocyanins and Flavones in Different-colored Flowers of Chrysanthemum[J]. Chin Bull Bot, 2010, 45(03): 327 -336 .
[9] . Phosphate_Stress Protein and Iron_Stress Protein in Plants[J]. Chin Bull Bot, 2001, 18(05): 571 -576 .
[10] ZHANG Da-Yong, JIANG Xin-Hua. An Ecological Perspective on Crop Prduction[J]. Chin J Plan Ecolo, 2000, 24(3): 383 -384 .