Chin Bull Bot ›› 2016, Vol. 51 ›› Issue (6): 764-773.doi: 10.11983/CBB16004

Previous Articles     Next Articles

Analysis of Differentially Expressed Genes in Suaeda liaotungensis NAC4 (SlNAC4)-transgenic Arabidopsis

Qianqian Li, Yang Jiao, Jingyang Yu, Qiuli Li*   

  1. College of Life Sciences, Liaoning Normal University/Key Laboratory of Plant Biotechnology of Liaoning Province, Dalian 116081, China
  • Received:2016-01-08 Revised:2016-06-15 Online:2016-12-02 Published:2016-11-01
  • Contact: Qiuli Li


Microarray assay was used for gene expression profiling of Suaeda liaotungensis NAC4 (SlNAC4)-transgenic Arabidopsis and the wild type. SlNAC4-transgenic Arabidopsis showed 3 094 differentially expressed genes as compared with the wild type. Gene ontology analysis revealed 195 differentially expressed genes related to abiotic stress and 47 related to growth and development, including MYB and WRKY transcription factor genes. KEGG analysis revealed the differentially expressed genes involved in 90 signaling pathways. The main signaling pathways were plant hormone signal transduction and brassinosteroid synthesis. The differential expression of selected genes was validated by qRT-PCR. SlNAC4 regulated the expression of downstream genes directly and indirectly, functioning in plant growth and development and abiotic stress tolerance.

CLC Number: 

  • Q945.78

[1]Kazuko Yamaguchi-Shinozaki, Kazuo Shinozaki.Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses[J]. [J].Annual Review of Plant Biology, 2006, 57:781-803
[2]Lifeng Zhao, Yibing Hu, Kang Chong.ARAG1,an ABA-responsive DREB gene,plays a role in seed germination and drought tolerance of rice[J].ANNALS OF BOTANY, 2010, 105(3):401-409
[3]Prashant Raghunath Shingote, Prashant Govindrao Kawar, Madhuri Chandrakant Pagariya.SoMYB18, a sugarcane MYB transcription factor improves salt and dehydration tolerance in tobacco[J][J].Acta Physiologiae Plantarum, 2015, 37:217-217
[4]Ligang Chen, Yu Song, Shujia Li, et al.The role of WRKY transcription factors in plant abiotic stresses[J][J].BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS [J], 2012, 1819(2):120-128
[5]Bouaziz D, Charfeddine M, Jbir R.Identification and functional characterization of ten AP2ERF genes in potato[J].Plant Cell Tiss Organ Cult, 2015, 123(1):155-172
[6]李小兰, 胡玉鑫, 杨星等.非生物胁迫相关转录因子的结构及功能[J].植物生理学报, 2013, 49(10):1009-1017
[7]Souer E, van Houwelingen A, Kloos D, et al.The No Apical Meristem Gene of Petunia Is Required for Pattern Formation in Embryos and Flowers and Is Expressed at Meristem and Primordia Boundaries[J].Cell, 1996, 85(2):159-170
[8]Aida M, Ishida T, Fukaki H, et al.Genes involved in organ separation in Arabidopsis: An analysis of the cup-shaped cotyledon mutant[J].plant cell, 1997, 9(6):841-857
[9]Zhong R, Demura T, Ye ZH .SND1, a NAC domain transcription factor, is a key regulator of secondary wall synthesis in fibers of Arabidopsis[J].[J].Plant Cell, 2006, 18:3158-3170
[10]Emily H.Grant,Takeshi Fujino,Eric P,et alCharacterization of NAC domain transcription factors implicated in control of vascular cell differentiation in Arabidopsis and Populus[J].Planta, 2010, 232(2):337-352
[11]、Yu-Jun Hao, Wei Wei, Qing-Xin Song, et al.Soybean NAC transcription factors promote abiotic stress tolerance and lateral root formation in transgenic plants[J][J].the plant journal, 2011, 68(2):302-313
[12]Movahedi A, Zhang Jx, Yin Tm,et al.Functional Analysis of Two Orthologous NAC Genes, CarNAC3, and CarNAC6 from Cicer arietinum, Involved in Abiotic Stresses in Poplar[J]. [J].Plant Mol Biol Rep, 2015, 33:1539-1551
[13]Xuanwen Yang, Xiaoyu Wang, Lu Ji, et al.Over of a Miscanthus lutarioriparius NAC gene MlNAC5 confers enhanced drought and cold tolerance in Arabidopsis[J].Plant Cell Reports, 2015, 34(6):943-958
[14]Xiao-lan Li, Xing Yang, Yu-xin Hu, et al.A novel NAC transcription factor from Suaeda liaotungensis Kenhanced transgenic Arabidopsis drought,salt,and cold stress tolerance[J].Plant Cell Reports, 2014, 33(5):767-778
[15]滕晓坤, 肖华胜.基因芯片与高通量测序技术前景分析[J].中国科学, 2008, 38(10):891-899
[16]Brown PO, Botstein D.Exploring the new world of the genome with DNA microarrays[J]. [J].nature genetics, 1999, 21:33-37
[17]宿明星.辽宁碱蓬SlNAC4、SlNAC10转录因子基因克隆及功能分析[D]., 2012, :-
[18]刘浩.拟南芥一个20G-Fe(Ⅱ)氧化还原酶在GA合成及其与ABA互作的功能分析[D]., 2011, :-
[19]Pauline A,Bariola, Gustavo C. MacIntosh, Pamela J. Green.. Regulation of S-Like Ribonuclease Levels in Arabidopsis. Antisense Inhibition of RNS1 or RNS2 Elevates Anthocyanin Accumulation[J]. [J].Plant Physiology, 1999, 119:331-342
[20]Vincent Sauveplane, Sylvie Kandel, Pierre-Edouard Kastner, et al.Arabidopsis thaliana CYP77A4 is the first cytochrome P450 able to catalyze the epoxidation of free fatty acids in plants[J].the FEBS Journal, 2009, 276(3):719-735
[21]Kazuko Yoshida, Rieko Iwasaka, Norimoto Shimada, et al.Transcriptional control of the dihydroflavonol 4-reductase multigene family in Lotus japonicus[J]. [J].J Plant Res, 2010, 123:801-805
[22]Caterina Mare, Elisabetta Mazzucotelli, Cristina Crosatti,et al.Hv-WRKY38: a new transcription factor involved in cold- and drought-response in barley[J][J].Plant Molecular Biology, 2004, 55:399-416
[23]Paul B,Larsen, Jesse D. Cancel.. Arecessive mutation in the RUB1-conjugating enzyme, RCE1, reveals a requirement for RUBmodification for control of ethylene biosynthesis and proper induction of basic chitinase and PDF1.2 in Arabidopsis[J], [J].The Plant Journal, 2004, 38:626-638
[24]Pauline A.Bariola2,Gustavo CMacIntosh,Pamela J. Green. Regulation of S-Like Ribonuclease Levels in Arabidopsis. Antisense Inhibition of RNS1 orRNS2 Elevates Anthocyanin Accumulation[J].Plant Physiologists, 1999, 119(1):331-342
[25]Chaonan Lia, Carl K-Y. Ngb, Liu-Min Fan.. MYB transcription factors, active players in abiotic stress signaling[J].[J].Environmental and Experimental Botany, 2015, 114:80-91
[26]Deena L.Rushton,Prateek Tripathi,Roel CRabara,et al. WRKY transcription factors: key components in abscisic acid signalling[J].plant biotechnology journal, 2012, 10(1):2-11
[27]Waadt R, Hsu PK, Schroeder JI.Abscisic acid and other plant hormones: Methods to visualize distribution and signaling[J].Bioessays, 2015, 37(12):1338-49
[28]Reitman, Sand Frankel S.A colorimetric method for the determination of serum glutamic oxalacetic oxalacetic and glutamic pyruvic transaminases[J].Am J Clin pathol, 1957, 38(5):23-
[29]赵毓橘.油菜素内酯研究进展[J][J].植物学通报, 1995, 12:30-34
[30]Sairam R K.Effect of homobor as sinolide application on plangmet abolismand grain yield under irrigated and moisture stress conditions of two wheat varieties[J]. [J].Plant Growth Regulation, 1994, 14:173-173

No related articles found!
Full text



[1] Yu Feng-lan;Wang Jing-ping;Li Jing-min and Shan Xue-qin. The Isolation and Identification of Sterols and Other Constituents from Seed Fat of Sapium sebiferum[J]. Chin Bull Bot, 1989, 6(02): 121 -123 .
[2] LI Al-Fen;CHEN Min amd ZHOU Bai-Cheng. Advances and Problems in Studies of Photosynthetic Pigment-Protein Complexes of Brown Algae[J]. Chin Bull Bot, 1999, 16(04): 365 -371 .
[3] CHEN Xiao-Mei and GUO Shun-Xing. Research Advances in Plant Disease Resistive Material[J]. Chin Bull Bot, 1999, 16(06): 658 -664 .
[4] LI Ji-Quan JIN You-Ju SHEN Ying-Bai HONG Rong. The Effect of Environmental Factors on Emission of Volatile Organic Compounds from Plants[J]. Chin Bull Bot, 2001, 18(06): 649 -656 .
[5] . [J]. Chin Bull Bot, 2005, 22(增刊): 157 .
[6] Jianxia Li, Chulan Zhang, Xiaofei Xia, Liangcheng Zhao. Cryo-sectioning Conditions and Histochemistry Comparison with Paraffin Sectioning[J]. Chin Bull Bot, 2013, 48(6): 643 -650 .
[7] JIANG Yang-Ming, CUI Wei-Hong, and DONG Qian-Lin. Comprehensive evaluation and analysis of tobacco planting environment based on space technology[J]. Chin J Plan Ecolo, 2012, 36(1): 47 -54 .
[8] Hu Cheng-biao, Zhu Hong-guang, Wei Yuan-lian. A Study on Microorganism and Biochemical Activity of Chinese-fir Plantation on Different Ecological Area in Guangxi[J]. Chin J Plan Ecolo, 1991, 15(4): 303 -311 .
[9] Hong-Xin SU Fan BAI Guang-Qi LI. Seasonal dynamics in leaf area index in three typical temperate montane forests of China: a comparison of multi-observation methods[J]. Chin J Plan Ecolo, 2012, 36(3): 231 -242 .
[10] AN Ran, GONG Ji-Rui, YOU Xin, GE Zhi-Wei, DUAN Qing-Wei, YAN Xin. Seasonal dynamics of soil microorganisms and soil nutrients in fast-growing Populus plantation forests of different ages in Yili, Xinjiang, China[J]. Chin J Plan Ecolo, 2011, 35(4): 389 -401 .