Chinese Bulletin of Botany ›› 2012, Vol. 47 ›› Issue (4): 395-404.doi: 10.3724/SP.J.1259.2012.00395

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The Movement Protein of Barley Yellow Dwarf Virus is a Suppressor of RNA Silencing

Yongmei Shen, Guofu Liu, Jijun Yang, Xiaohui Huo, Xuesong Cao*   

  1. School of Life Sciences, Liaocheng University, Liaocheng 252059, China
  • Received:2011-10-28 Revised:2012-03-30 Online:2012-07-26 Published:2012-07-01
  • Contact: Xuesong Cao

Abstract: The genome of barley yellow dwarf virus (BYDV), a member of the Luteoviridae family, contains 6 open reading frames (ORFs). We cloned the 6 ORFs into plasmid of pWEIMING101 and electroporated them into Agrobacterium tumefaciens for transient coexpression of BYDV ORFs and GFP in Nicotiana benthamiana line 16c, a transgenic GFP plant. Northern blot analysis revealed the movement protein (MP) encoded by BYDV-PAV ORF4 as a suppressor of both local and systemic GFP RNA silencing and RNA silencing triggered by double-stranded GFP RNA. The fluorescent intensity was associated with steady state levels of GFP mRNAs and its degradation product of siRNAs. The nuclear localization signal in the N terminus of the MP and amino acid 5 and 6 were the functional domain and motif, respectively, essential for inhibiting RNA silencing. Transient expression of BYDV-MP alone in infiltrated tissue resulted in cell death.

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