Chin Bull Bot ›› 2012, Vol. 47 ›› Issue (4): 422-426.doi: 10.3724/SP.J.1259.2012.00422

Previous Articles     Next Articles

A Method to Improve Sample Preparation for Observing Epidermis of Highly Lignified Leaf

Qinghan Wu1,2, Qingfang Zhao2, Hui Zhu1, Ruijun Ma1*   

  1. 1Institute of Plant Resources, Hanshan Normal University, Chaozhou 521041, China;

    2College of Life Sciences, Northwest Normal University, Lanzhou 730070, China
  • Received:2011-11-23 Revised:2012-02-29 Online:2012-07-26 Published:2012-07-01
  • Contact: Ruijun Ma E-mail:ruijunma2003@yahoo.com.cn

Abstract: We have developed an efficient leaf-burning method to observe epidermal cells of tea which are highly lignified in cell walls. With this method, the clear shape of the upper and lower epidermis cells can be observed after several steps of burning and rinsing. After treatment, the upper and lower epidermis cells can automatically separate and hyalinize. This method is easy to use, saves time, distinguishes the upper from the lower side of plant epidermis and has high reliability and stability. The epidermal characteristics of the lignified leaves can be accurately and quickly determined for botany and ecology research.

陈佰鸿, 李新生, 曹孜义, 姚庆荣(2004). 一种用透明胶带粘取叶片表皮观察气孔的方法. 植物生理学通讯 40, 215-218.
崔宏安, 柳延琴, 白红霞(2007). 观察气孔的一种新方法. 西北农业学报 16, 305-306.
Koesis M, Darok J, Borhide A(2004). Comparative leaf anatomy and morphology of some neotropical Rondeletia (Rubiaceae) species. Plant Systematics and Evolution 248, 205-218.
林月惠(1996). 气孔复型法. 植物杂志 (4), 39.
税玉民, 李启任, 黄素华(1999). 云南秋海棠属叶表皮及毛被的扫描电镜观察. 云南植物研究21, 309-316.
石硕, 贺学礼, 祝东立(2008). 不同海拨高度上两种蒿属植物叶表皮结构比较研究. 河北农业大学学报3, 42-47.
吴杰, 梁敏婷, 王小菁, 彭建宗(2008). 琼脂糖印迹法以:观察植物表皮细胞的一种简易方法. 植物学通报25, 332-336.
于慧, 夏念和, 胡晓颖(2011). 中国鼠李科植物的叶表皮微形态. 热带亚热带植物学报19, 16-25.
杨虎彪, 李晓霞, 罗丽娟(2009). 一种禾本科植物鲜叶下表皮制片方法. 植物研究29, 242-244.
张志耘, 卢宝荣, 温洁(1998). 稻属叶表皮结构特征及其系统学意义. 植物分类学报36, 8-18.
No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] . [J]. Chin Bull Bot, 1994, 11(专辑): 19 .
[2] Xiao Xiao and Cheng Zhen-qi. Chloroplast 4.5 S ribosomol DNA. II Gene and Origin[J]. Chin Bull Bot, 1985, 3(06): 7 -9 .
[3] CAO Cui-LingLI Sheng-Xiu. Effect of Nitrogen Level on the Photosynthetic Rate, NR Activity and the Contents of Nucleic Acid of Wheat Leaf in the Stage of Reproduction[J]. Chin Bull Bot, 2003, 20(03): 319 -324 .
[4] SONG Li-Ying TAN Zheng GAO Feng DENG Shu-Yan. Advances in in vitro Culture of Cucurbitaceae in China[J]. Chin Bull Bot, 2004, 21(03): 360 -366 .
[5] . [J]. Chin Bull Bot, 1994, 11(专辑): 76 .
[6] LI Jun-De YANG Jian WANG Yu-Fei. Aquatic Plants in the Miocene Shanwang Flora[J]. Chin Bull Bot, 2000, 17(专辑): 261 .
[7] XU Jing-Xian WANG Yu-Fei YANG Jian PU Guang-Rong ZHANG Cui-Fen. Advances in the Research of Tertiary Flora and Climate in Yunnan[J]. Chin Bull Bot, 2000, 17(专辑): 84 -94 .
[8] Sun Zhen-xiao Xia Guang-min Chen Hui-min. Karyotype Analysis of Psathyrostachys juncea[J]. Chin Bull Bot, 1995, 12(01): 56 .
[9] . [J]. Chin Bull Bot, 1994, 11(专辑): 8 -9 .
[10] Yunpu Zheng;Jiancheng Zhao * ;Bingchang Zhang;Lin Li;Yuanming Zhang . Advances on Ecological Studies of Algae and Mosses in Biological Soil Crust[J]. Chin Bull Bot, 2009, 44(03): 371 -378 .