Chinese Bulletin of Botany ›› 2012, Vol. 47 ›› Issue (1): 36-43.DOI: 10.3724/SP.J.1259.2012.00036

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Anatomy of Papilla Cells of a Female Sterile Mutant FS-M1 in Brassica napus

Chunhong Li, Sanxiong Fu, Xinjun Chen, Cunkou Qi*   

  1. Nanjing Sub-center of National Rapeseed Development Center, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
  • Received:2011-07-05 Revised:2011-11-21 Online:2012-01-01 Published:2012-01-16
  • Contact: Cunkou Qi

Abstract: The female sterile mutant FS-M1 was isolated from spontaneous mutation of Brassica napus ‘Ningyou10’. To understand the cellular mechanism of female sterility, we investigated the pollination response and ultrastructure of FS-M1 papilla cells by fluorescence microscope, scanning electron and transmission electron microscopy. Pollen grains could adhere to and germinate to produce pollen tubes on FS-M1 papilla cells, but the pollen tubes could not penetrate into papilla cells. FS-M1 papilla cells became withered and degenerated quickly after flowering. During the degeneration, the number of organelles was significantly decreased; vacuolization was obvious; some organelles such as dictyosome, endoplasmic reticulum, and mitochondria gradually became misshapen and degenerated. Therefore, female sterility in FS-M1 may have resulted from defects in papilla cells.

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