Chin Bull Bot ›› 2018, Vol. 53 ›› Issue (6): 793-800.doi: 10.11983/CBB17215

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

Clarifying the Taxonomic Status of Dryopteris yenpingensis based on Materials from the Type Locality

Shu Jiangping1,2, Luo Junjie1, Wei Hongjin1, Yan Yuehong1,2,3,*()   

  1. 1Shanghai Chenshan Plant Science Research Center, Shanghai Chenshan Botanical Garden, Chinese Academy of Sciences, Shanghai 201602, China
    2Shanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai 201602, China
    3Eastern China Conservation Center for Wild Endangered Plant Resources, State Forestry Administration, Shanghai 201602, China
  • Received:2017-11-13 Online:2018-12-05 Published:2018-11-01
  • Contact: Yan Yuehong E-mail:yhyan@sibs.ac.cn

Abstract:

Calculating the number of species on earth has always been an issue of extreme concern, and suspicious species are among the most important obstacles to solve. Presently, the type specimen is the only criteria for identifying the suspicious species but is difficult to observe and study effectively because of its special taxonomic position. Therefore, the mostly effective way is to search the original information for suspicious species in the type locality. As molecular biological method, DNA barcoding, has been widely used in species classification and identification and can be used to identify a suspicious species accurately and quickly. In this study, a suspicious species, Dryopteris yenpingensis, was used as an example. We used DNA barcode analysis with the molecular data for the material from the type locality and combined morphological traits to clarify the doubtful taxonomic status of D. yenpingensis. The results supported D. yenpingensis and D. liboensis as the same species, and D. liboensis should be treated as a synonym. This study provides a reference for clarifying the taxonomic status of other suspicious species.

Key words: Dryopteris yenpingensis, type locality, DNA barcode, taxonomically suspicious species, biodiversity

Table 1

The information of the specimens in present study"

Voucher number Species name Collection site
ZXC7305 Dryopteris liboensis Guizhou, China
ZXC7273 D. liboensis Guizhou, China
WYD043 D. liboensis Guangdong, China
WYD066 D. integriloba Guangdong, China
JSL5149 D. yenpingensis Fujian, China
JSL3693 D. integriloba Guangxi, China
JSL5083 D. integriloba Guangxi, China

Figure 1

The phylogenetic tree constructed by Bayesian inference (A) and neighbor-joining (B) methods"

Figure 2

The DNA barcoding gap based on rbcL"

Figure 3

The plants, spores and scales of Dryopteris yenpingensis(A) Plants; (B) Frond; (C) Rhizome; (D) Spore; (E) Scale"

Figure 4

The geographic distribution of Dryopteris yenpingensis"

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