Chinese Bulletin of Botany ›› 2011, Vol. 46 ›› Issue (5): 560-568.doi: 10.3724/SP.J.1259.2011.00560

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Construction and Activity Analysis of an Antibiotic-inducible Promoter

Weiwei Liu, Hailei Zhang, Caifeng Liu, Xiaochun Ge*   

  1. Department of Biochemistry and Molecular Biology, Institute of Plant Science, School of Life Sciences, Fudan University, Shanghai 200433, China
  • Received:2011-01-31 Revised:2011-05-13 Online:2011-09-01 Published:2011-09-01
  • Contact: Xiaochun Ge

Abstract: Poly ADP-ribose polymerase (PARP) is induced specifically by genotoxin. We cloned the 2 179 bp promoter sequence of Arabidopsis AtPARP1 gene into a pAKK687 vector to drive the expression of a β-glucuronidase (GUS) reporter gene for Arabidopsis transformation. GUS staining revealed the GUS reporter gene expressed only in the basal root of 3 to 5-day-old seedlings and in the anther at early flowering stage. An amount of 1.5 μg·mL–1 bleomycin combined with 22 μg·mL–1 mitomycin could strongly induce the expression of the GUS reporter gene, especially in young seedlings and young siliques of Arabidopsis. An amount of 1 μg·mL–1 bleomycin was enough to induce the promoter and had no adverse effects on Arabidopsis seedling growth. The AtPARP1 promoter may be a new antibiotic-inducible promoter with application potential.

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