Chin Bull Bot ›› 2010, Vol. 45 ›› Issue (04): 460-465.doi: 10.3969/j.issn.1674-3466.2010.04.009

• 技术方法 • Previous Articles     Next Articles

orting of early developmental non-hair cells in root by flow cytometry in Arabidopsis thaliana

Fei Li&; Yong Hu&; Fan Wang; Zhen Zhang; Xianglin Liu*; Sulan Bai*; Yikun He   

  1. College of Life Sciences, Capital Normal University, Beijing 100048
  • Received:2010-01-18 Online:2010-09-26 Published:2010-04-30
  • Contact: Xianglin Liu;Sulan Bai

Abstract: We have developed a method for sorting specific cells in plant. Here, WER::GFP transgenic lines in Arabidopsis thaliana were as the experimental material; laser scanning confocal microscopy was used to identify the expression location of GFP; enzymatic digestion was applied for preparation of root tip protoplasts; fluorescence activated cell sorting (FACS) was applied to collect GFP positive cells; and then the total RNA was extracted from these cells. The results showed that only the early developmental non-root hair cells in the root epidermis express GFP in Wer::GFP transgenic lines; larger number of root tip protoplasts were obtained by using enzymatic digestion; and then GFP positive cells were collected successfully by FACS. The RNA extracted from these specific cells can be used for gene expression profiling. The method for sorting specific cells had been established successfully.

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