Chin Bull Bot ›› 2016, Vol. 51 ›› Issue (5): 577-585.doi: 10.11983/CBB16117

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Association of CBF3 Expression and ROS Content Under Low Temperature among Natural Populations of Arabidopsis thaliana in China

Juqing Kang1,2*, Daipeng Zhang2   

  1. 1College of Life Sciences, Shaanxi Normal University, Xi’an 710119, China; 

    2College of Life Sciences, Peking University, Beijing 100871, China
  • Received:2016-05-30 Revised:2016-06-20 Online:2016-09-27 Published:2016-09-01
  • Contact: Juqing Kang


Reactive oxygen species (ROS), a by-product of photosynthesis and respiration, is increased under many stress conditions, which would result in the peroxidation of the cytomembrane and damage of the photosystem II reaction center. Soluble carbohydrates such as raffinose family oligosaccharide (RFOs) could respond to stress and might be involved in scavenging hydroxyl radicals. Previous work demonstrated that the freezing tolerance and RFO content of populations of Arabidopsis thaliana along the Yangtze River in China were decreased as compared to the Col wild type because of a natural variation of C-repeat binding factor 3 (CBF3). In this study, we examined the transcription profile of 4 populations along the Yangtze River under cold conditions and found a different pathway of scavenging hydroxyl radicals in these 4 populations as compared with the Col ecotype. Also the concentration of ROS was greater in these 4 populations than in the Col ecotype. When the cold-inducible allele of CBF3 from the Col ecotype was transformed into the CQtlx population, from the highest warmest habitat with the lowest freezing tolerance, the freezing tolerance of transgenic plants was improved significantly as compared with non-transgenic CQtlx plants. At the same time, the concentration of RFOs was increased, but that of ROS was decreased. Thus, under cold conditions, CBF3 could induce synthesis of RFOs, probably to scavenge hydroxyl radicals. The higher concentration of ROS and lower freezing tolerance in populations along the Yangtze River as compared with the Col ecotype might be explained at least in part by the natural variation of CBF3 in these populations.

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[1] . [J]. Chin Bull Bot, 1994, 11(专辑): 19 .
[2] Xiao Xiao and Cheng Zhen-qi. Chloroplast 4.5 S ribosomol DNA. II Gene and Origin[J]. Chin Bull Bot, 1985, 3(06): 7 -9 .
[3] CAO Cui-LingLI Sheng-Xiu. Effect of Nitrogen Level on the Photosynthetic Rate, NR Activity and the Contents of Nucleic Acid of Wheat Leaf in the Stage of Reproduction[J]. Chin Bull Bot, 2003, 20(03): 319 -324 .
[4] SONG Li-Ying TAN Zheng GAO Feng DENG Shu-Yan. Advances in in vitro Culture of Cucurbitaceae in China[J]. Chin Bull Bot, 2004, 21(03): 360 -366 .
[5] . [J]. Chin Bull Bot, 1994, 11(专辑): 76 .
[6] LI Jun-De YANG Jian WANG Yu-Fei. Aquatic Plants in the Miocene Shanwang Flora[J]. Chin Bull Bot, 2000, 17(专辑): 261 .
[7] XU Jing-Xian WANG Yu-Fei YANG Jian PU Guang-Rong ZHANG Cui-Fen. Advances in the Research of Tertiary Flora and Climate in Yunnan[J]. Chin Bull Bot, 2000, 17(专辑): 84 -94 .
[8] Sun Zhen-xiao Xia Guang-min Chen Hui-min. Karyotype Analysis of Psathyrostachys juncea[J]. Chin Bull Bot, 1995, 12(01): 56 .
[9] . [J]. Chin Bull Bot, 1994, 11(专辑): 8 -9 .
[10] Yunpu Zheng;Jiancheng Zhao * ;Bingchang Zhang;Lin Li;Yuanming Zhang . Advances on Ecological Studies of Algae and Mosses in Biological Soil Crust[J]. Chin Bull Bot, 2009, 44(03): 371 -378 .