Chin Bull Bot ›› 2013, Vol. 48 ›› Issue (3): 344-353.doi: 10.3724/SP.J.1259.2013.00344

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Use of Degradome Sequencing in Study of Plant MicroRNAs

Miao Dong1, Yue Huang1, Wenduo Chen1, Tao Xu1*, Qiulei Lang2*   

  1. 1College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China

    2LianChuan-Biotechnology, Hangzhou 310018, China
  • Received:2012-09-14 Revised:2012-11-14 Online:2013-06-21 Published:2013-05-01
  • Contact: Tao Xu,Qiulei Lang E-mail:xutao1998cn@yahoo.com.cn
  • Supported by:

    NSFC

Abstract: MicroRNA (miRNA) chip and sequencing have been used to detect miRNAs in species. With the discovery of more miRNAs, miRNA target genes are the key to study miRNA biological function. Finding miRNA target genes mainly relies on computational prediction, AGO protein co-immunoprecipitation and luciferase. Deep sequencing, or degradome sequencing, can be used for high-throughput identification of targets of miRNAs. The method includes deep sequencing, bioinformatic analysis and 5'-rapid amplification of cDNA ends. It has been successfully used for global identification of miRNA-target RNA pairs in Arabidopsis thaliana, Oryza sativa and Physcomitrella patens. This paper reviews research progress in degradome sequencing in plant miRNA target study and discusses trends in further application of degradome sequencing.

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[1] Lu Zhong-shu. Plant Growth Regutators in Relation to Plant Water Status[J]. Chin Bull Bot, 1985, 3(04): 1 -6 .
[2] Li Da Jue;Han Yun-zhou and Wan Li-ping. Studies on Germplasm Collections of Carthamus tinctorius IV Screening of the characterization of Seed Domancy[J]. Chin Bull Bot, 1990, 7(02): 50 -52 .
[3] . [J]. Chin Bull Bot, 1999, 16(增刊): 45 -46 .
[4] Yang Hong-yuan. Basic Principle and Method of Fluorescence Microscopy[J]. Chin Bull Bot, 1984, 2(06): 45 -48 .
[5] LU Jin-Yao;LUO Ai-Ling and LIANG Zheng. Some Improvement of TD-PAGE Technology[J]. Chin Bull Bot, 1998, 15(03): 69 -72 .
[6] LI Ling-Hao and CHEN Zuo-Zhong. The Global Carbon Cycle in Grassland Ecosystems and Its Responses to Global Change I . Carbon Flow Compartment Model, Inputs and Storage[J]. Chin Bull Bot, 1998, 15(02): 14 -22 .
[7] Huanhuan Xu, Jian Kang, Mingxiang Liang. Research Advances in the Metabolism of Fructan in Plant Stress Resistance[J]. Chin Bull Bot, 2014, 49(2): 209 -220 .
[8] . [J]. Chin Bull Bot, 2013, 48(1): 4 -5 .
[9] . [J]. Chin Bull Bot, 1996, 13(专辑): 45 .
[10] SHU Qun-Fang;ZHOU Lu;LI Wen-Bin;ZHANG LI-Ming and SUN Yong-Ru. Study on Gel Electrophoresis of Protein from Plant and Our Improved Methods[J]. Chin Bull Bot, 1998, 15(06): 73 -78 .