Chin Bull Bot ›› 2011, Vol. 46 ›› Issue (2): 179-188.doi: 10.3724/SP.J.1259.2011.00179

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Morphological and Phylogenetic Analysis of Karlodinium veneficum Isolated from the East China Sea in China

Hongxia Wang, Douding Lu*, Haiyan Huang, Xinfeng Dai, Ping Xia   

  1. Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, China
  • Received:2010-07-23 Revised:2010-11-07 Online:2011-05-09 Published:2011-03-01
  • Contact: Dou-Ding LU

Abstract: Karlodinium veneficum (D. Ballantine) J. Larsen (LAMB090611), isolated from the East China Sea of Zhejiang province, was reported for the first time in China. The strain of LAMB090611 was described on the basis of morphological characteristics, and molecular phylogenetics. The results of this study were acquired by light microscopy, fluorescence microscopy, scanning electron microscopy and molecular sequencing. The cells have a mean length of 14.2±1.8 μm (range 11.1–18.7 μm) and mean width of 10.8±1.5 μm (range 8.2–14.7 μm). The size of epicone is approximately the same as that of hypocone. The cell has a short, straight apical groove and distinct ventral pore, with usually 2 or 4 chloroplasts distributed irregularly in the cell. The sulcus extends onto the epicone. The cingulum displacement is about 28%–38% of the body length. The nucleus is in middle of the cell or in the hypocone. A high density of this species results in harmful algal bloom. Therefore, the precise identification and detection of the causative species are important for monitoring and mitigation of such blooms.

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