Chin Bull Bot ›› 2009, Vol. 44 ›› Issue (04): 491-496.doi: 10.3969/j.issn.1674-3466.2009.04.011
• 技术方法 •
Amei Yu1, Zhixian Su2*, Liqiang Wang3, Zhirong Fang4, Lijuan Zou1
Three factors (media type, gibberellic acid [GA3] and active carbon) affecting the embryo germination of Davidia involucrata were optimized by use of a uniform design and analyzed by regression analysis . Then a system for micropropagating D. involucrata was established from the embryos . From regression equations, the optimal conditions for embryo germination were the addition of 2.85 mg.L-1 GA3 and 0.25 g.L-1 activated carbon to 1/2 MS medium containing 0.5 mg.L-1 IBA and 1 mg.L-1 6-BA. The germination rate of embryo was 87.72%, without significant deviation to the predicted value. The optimal conditions for shoot induction and multiplication were WPM medium supplemented with 0.1 mg.L-1 NAA and 2.0 mg.L-1 6-BA; the mean shoots obtained per explant was 4.34. An amount of 90% of shoots rooted on rooting medium, and 71% plants survived after transplanting.
Amei Yu;Zhixian Su*;Liqiang Wang;Zhirong Fang;Lijuan Zou. Embryo Germination and Rapid Propagation of Rare and Endangered Plant Davidia involucrata[J].Chin Bull Bot, 2009, 44(04): 491-496.
Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks