Chin Bull Bot ›› 2009, Vol. 44 ›› Issue (04): 491-496.doi: 10.3969/j.issn.1674-3466.2009.04.011

• 技术方法 • Previous Articles     Next Articles

Embryo Germination and Rapid Propagation of Rare and Endangered Plant Davidia involucrata

Amei Yu1, Zhixian Su2*, Liqiang Wang3, Zhirong Fang4, Lijuan Zou1   

  1. 1Key Laboratory of Environmental and Biodiversity Conservation, China West Normal University, Nanchong 637002, China2Mianyang Normal College, Mianyang 621000, China
  • Received:2008-07-28 Revised:2008-11-24 Online:2009-07-01 Published:2009-07-01
  • Contact: Zhixian Su

Abstract: Three factors (media type, gibberellic acid [GA3] and active carbon) affecting the embryo germination of Davidia involucrata were optimized by use of a uniform design and analyzed by regression analysis . Then a system for micropropagating D. involucrata was established from the embryos . From regression equations, the optimal conditions for embryo germination were the addition of 2.85 mg.L-1 GA3 and 0.25 g.L-1 activated carbon to 1/2 MS medium containing 0.5 mg.L-1 IBA and 1 mg.L-1 6-BA. The germination rate of embryo was 87.72%, without significant deviation to the predicted value. The optimal conditions for shoot induction and multiplication were WPM medium supplemented with 0.1 mg.L-1 NAA and 2.0 mg.L-1 6-BA; the mean shoots obtained per explant was 4.34. An amount of 90% of shoots rooted on rooting medium, and 71% plants survived after transplanting.

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