Chin Bull Bot ›› 2009, Vol. 44 ›› Issue (04): 491-496.doi: 10.3969/j.issn.1674-3466.2009.04.011

• 技术方法 • Previous Articles     Next Articles

Embryo Germination and Rapid Propagation of Rare and Endangered Plant Davidia involucrata

Amei Yu1, Zhixian Su2*, Liqiang Wang3, Zhirong Fang4, Lijuan Zou1   

  1. 1Key Laboratory of Environmental and Biodiversity Conservation, China West Normal University, Nanchong 637002, China2Mianyang Normal College, Mianyang 621000, China
  • Received:2008-07-28 Revised:2008-11-24 Online:2009-07-01 Published:2009-07-01
  • Contact: Zhixian Su

Abstract: Three factors (media type, gibberellic acid [GA3] and active carbon) affecting the embryo germination of Davidia involucrata were optimized by use of a uniform design and analyzed by regression analysis . Then a system for micropropagating D. involucrata was established from the embryos . From regression equations, the optimal conditions for embryo germination were the addition of 2.85 mg.L-1 GA3 and 0.25 g.L-1 activated carbon to 1/2 MS medium containing 0.5 mg.L-1 IBA and 1 mg.L-1 6-BA. The germination rate of embryo was 87.72%, without significant deviation to the predicted value. The optimal conditions for shoot induction and multiplication were WPM medium supplemented with 0.1 mg.L-1 NAA and 2.0 mg.L-1 6-BA; the mean shoots obtained per explant was 4.34. An amount of 90% of shoots rooted on rooting medium, and 71% plants survived after transplanting.

No related articles found!
Full text



[1] Kang Le. The Chemical Defenses of plants to phytophagous Insects[J]. Chin Bull Bot, 1995, 12(04): 22 -27 .
[2] HUANG Kai-Yao;GUO Hou-Liang and YI Ping. Effects of Salt Stress on Cell Structure and N2 Fixation in Blue-Green Alga Anabaena cylindrica[J]. Chin Bull Bot, 1998, 15(03): 54 -56 .
[3] Zhang Jing-tan. Abbreviations for Some Commonly Used Term[J]. Chin Bull Bot, 1985, 3(01): 57 -58 .
[4] DU Gui-Sen;ZANG Yu-Long and WANG Mei-Zhi. Study on Spore Morphology of 6 Species of The Family Pottiaceae in China[J]. Chin Bull Bot, 1998, 15(03): 57 -60 .
[5] TIAN Xin-Zhi. On Plant Illustration and Artistic Drawing and Painting[J]. Chin Bull Bot, 1999, 16(04): 470 -476 .
[6] DUAN Hai-Yan WANG Yun-Hua XU Fang-Sen. Advance on Genetic Aspects of Phosphorus Efficiency in Plants[J]. Chin Bull Bot, 2002, 19(04): 432 -438 .
[7] LI Xiu-Lan WU Cheng DENG Xiao-Jian YANG Zhi-Rong. Plant Height Genes and Their Progress of Molecular Biology Research in Rice[J]. Chin Bull Bot, 2003, 20(03): 264 -269 .
[8] LIU Hong-Tao LI Bing ZHOU Ren-Gang. Calcium_calmodulin Signal Transduction Pathway and Environment Stimulation[J]. Chin Bull Bot, 2001, 18(05): 554 -559 .
[9] Renyi Gui;Yadi Liu;Xiaoqin Guo;Haibao Ji;Yue Jia;Mingzeng Yu;Wei Fang*. Effects of Dose of 137Cs-γ Irradiation on Chlorophyll Fluorescence Parameters for Leaves of Seedlings of Phyllostachys heterocycla ‘Pubescens’[J]. Chin Bull Bot, 2010, 45(01): 66 -72 .
[10] Sanxiong Fu;Cunkou Qi*. Identification of Genes Differentially Expressed in Seeds of Brassica napus Planted in Nanjing and Lhasa by Arabidopsis Microarray[J]. Chin Bull Bot, 2009, 44(02): 178 -184 .