Chinese Bulletin of Botany ›› 2009, Vol. 44 ›› Issue (04): 484-490.DOI: 10.3969/j.issn.1674-3466.2009.04.010

• 技术方法 • Previous Articles     Next Articles

Histochemical Study of b-glucuronidase Activity in Transgenic Birch

Fansuo Zeng1, 2, Jingjing Qian1, Jun Kang1, Hongyan Wang1, Yizhou Wang1, Yaguang Zhan1, 2   

  1. 1College of Life Sciences, Northeast Forestry University, Harbin 150040, China ; 2 Key Laboratory of Forest Tree Improvement and Biotechnology of Ministry of Education, Northeast Forestry University, Harbin 150040, China
  • Received:2008-10-15 Revised:2008-11-26 Online:2009-07-01 Published:2009-07-01
  • Contact: Yaguang Zhan


To determine the copy number of the transgene, the single- restriction enzyme Pst I was used to digest the genomic DNA. Southern blot analys is of transgenic birch plants indicated that the GUS gene had been integrated into the birch genome. Among the 11 transgenic birch lines, the copy number of GUS varied from1 to 4. The expression of the GUS gene w as studied by his tochemic al staining and spectrophotometry . His tochemical staining with X-gluc showed the GUS gene silenced in TP30 and TP96 plants , and that significant dif ferences of GUS activity was observed in other transgenic plants, with GUS activity remarkably high in TP23, TP36 and TP71 plants. However, the copy number and expression of the GUS gene were not significantly related.