Chinese Bulletin of Botany ›› 2009, Vol. 44 ›› Issue (02): 230-235.DOI: 10.3969/j.issn.1674-3466.2009.02.013
• 技术方法 • Previous Articles Next Articles
Hubiao Yang, Xiaoxia Li, Lijuan Luo*
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Paraffin sectioning plays an important role in plant science research. It is used widely in histology to observe and explain microstructure and provides a credible method for development of plant histology. This study aimed to investigate the limitations in traditional paraffin sectioning, such as the complex and time-consuming traditional protocol for improved measure. Xylene, a kind of neurotoxicant, is often used as a dewaxing and clearing chemical in the materials-preparation process. We substituted histolene (C1OH16), van-clear, oil of turpentine and n-butanol for xylene, to avoid a toxic effect. We improved the method to simplify the process of paraffin sectioning, shorten the cycle of the experiment and obtain quality slides.
Hubiao Yang;Xiaoxia Li;Lijuan Luo*. An Improved Clearing and De-waxing Method for Plant Paraffin Sectioning[J]. Chinese Bulletin of Botany, 2009, 44(02): 230-235.
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URL: https://www.chinbullbotany.com/EN/10.3969/j.issn.1674-3466.2009.02.013
https://www.chinbullbotany.com/EN/Y2009/V44/I02/230