Chin Bull Bot ›› 2009, Vol. 44 ›› Issue (02): 230-235.doi: 10.3969/j.issn.1674-3466.2009.02.013
• 技术方法 •
Hubiao Yang, Xiaoxia Li, Lijuan Luo*
Paraffin sectioning plays an important role in plant science research. It is used widely in histology to observe and explain microstructure and provides a credible method for development of plant histology. This study aimed to investigate the limitations in traditional paraffin sectioning, such as the complex and time-consuming traditional protocol for improved measure. Xylene, a kind of neurotoxicant, is often used as a dewaxing and clearing chemical in the materials-preparation process. We substituted histolene (C1OH16), van-clear, oil of turpentine and n-butanol for xylene, to avoid a toxic effect. We improved the method to simplify the process of paraffin sectioning, shorten the cycle of the experiment and obtain quality slides.
Hubiao Yang;Xiaoxia Li;Lijuan Luo*. An Improved Clearing and De-waxing Method for Plant Paraffin Sectioning[J].Chin Bull Bot, 2009, 44(02): 230-235.
Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks