Chin Bull Bot ›› 2009, Vol. 44 ›› Issue (02): 230-235.doi: 10.3969/j.issn.1674-3466.2009.02.013

• 技术方法 • Previous Articles     Next Articles

An Improved Clearing and De-waxing Method for Plant Paraffin Sectioning

Hubiao Yang, Xiaoxia Li, Lijuan Luo*   

  1. College of Horticulture and Garden Architecture, Hainan University, Danzhou 571737, China
  • Received:2008-05-26 Revised:2008-10-05 Online:2009-03-01 Published:2009-03-01
  • Contact: Lijuan Luo

Abstract:

Paraffin sectioning plays an important role in plant science research. It is used widely in histology to observe and explain microstructure and provides a credible method for development of plant histology. This study aimed to investigate the limitations in traditional paraffin sectioning, such as the complex and time-consuming traditional protocol for improved measure. Xylene, a kind of neurotoxicant, is often used as a dewaxing and clearing chemical in the materials-preparation process. We substituted histolene (C1OH16), van-clear, oil of turpentine and n-butanol for xylene, to avoid a toxic effect. We improved the method to simplify the process of paraffin sectioning, shorten the cycle of the experiment and obtain quality slides.

No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] . Studies on physiology of floral induction and zearalenone[J]. Chin Bull Bot, 1995, 12(专辑3): 18 -29 .
[2] Wu Jian-feng. Lichen Ptant in Langskan Area Nantong[J]. Chin Bull Bot, 1993, 10(01): 62 .
[3] . [J]. Chin Bull Bot, 1998, 15(专辑): 35 -38 .
[4] . [J]. Chin Bull Bot, 2000, 17(05): 478 .
[5] WANG Zheng-Feng ZHANG Jun-Li LI Ming-Guang WANG Bo-Sun HE Xing-Jin PENG Shao-Lin. Advances of Plant Molecular Ecology (Ⅰ)—— Genetic Structure and Hybridization[J]. Chin Bull Bot, 2001, 18(06): 635 -642 .
[6] Zhao Yu-hua. Effect of the Plant Cold-resister on Overcoming Rice Seedling Decay in Low Temperature Stress in our County[J]. Chin Bull Bot, 1994, 11(特辑): 97 -99 .
[7] . [J]. Chin Bull Bot, 2015, 50(6): 765 -767 .
[8] Danlong Jing, Jiang Ma, Bo Zhang, Yiyang Han, Zhixiong Liu, Faju Chen. Expression Analysis of MwAG in Different Organs and Developmental Stages of Magnolia wufengensis[J]. Chin Bull Bot, 2013, 48(2): 145 -150 .
[9] Li Xiang-gan. The Population Characteristics and determination of Biomass in the Quercus acutissima Forest Community on Mount Lao[J]. Chin J Plan Ecolo, 1987, 11(1): 21 -31 .
[10] Jin Zhen-zhou. The Characteristics and Utilization of Shrub-grasslands in Tropical and Subtropical Mountains of Yunnan[J]. Chin J Plan Ecolo, 1986, 10(2): 81 -89 .