Chin Bull Bot ›› 2015, Vol. 50 ›› Issue (1): 40-46.doi: 10.3724/SP.J.1259.2015.00040

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Effect of Different Times of UV-B Radiation on Seedling Growth of Arabidopsis thaliana

Wen Han, Rong Han*   

  1. College of Life Sciences, Shanxi Normal University, Linfen 041004, China
  • Received:2013-11-13 Accepted:2014-03-12 Online:2015-04-09 Published:2015-01-01
  • Contact: Han Rong E-mail:hhwrsl@yahoo.com.cn
  • About author:

    ? These authors contributed equally to this paper

Abstract:

We treated Columbia-0 wild-type Arabidopsis thaliana seedlings with enhanced UV-B radiation (16.67 μW·cm-2 radiant power) at 0.5, 1, 1.5, 2, 2.5, and 3 h. We observed leaf morphological characters and detected root length, leaf chlorophyll content, soluble protein content, malondialdehyde (MDA) concentration, superoxide dismutase (SOD) activity, catalase (CAT) activity and chlorophyll fluorescence characteristics. With a short period of UV-B radiation, roots were elongated and chlorophyll content and soluble protein content were increased in leaf, but prolonged UV-B radiation inhibited the elongated root length; caused decreased leaf chlorophyll content, soluble protein content and chlorophyll fluorescence characteristics such as Fv/Fm and qP; promoted MDA concentration; and increased SOD and CAT activity and qN over time. With 16.67 μW·cm-2 radiation, the optimal time of UV-B radiation was 1.5 h. If UV-B radiation acts as a kind of environmental stress, the extent of damage to the plant was restricted, and the plant would have to adapt to UV-B radiation to let the damage reach a minimum when the stress level reached the limitation.

Figure 1

The leaf morphological character of Arabidopsis thaliana with different periods of time of UV-B radiation (A) Control (CK); (B)-(G) B1-B6 treatments, with enhanced UV-B radiation at 0.5, 1, 1.5, 2, 2.5, and 3 h, respectively"

Figure 2

The root length of Arabidopsis thaliana with different periods of time of UV-B radiation CK: Control; B1-B6 see Figure 1"

Figure 3

The leaf chlorophyll content of Arabidopsis thaliana with different periods of time of UV-B radiation CK: Control; B1-B6 see Figure 1"

The soluble protein content of Arabidopsis thaliana with different periods of time of UV-B radiation CK: Control; B1-B6 see Figure 1"

The malondialdehyde concentration of Arabidopsis thaliana with different periods of time of UV-B radiation CK: Control; B1-B6 see Figure 1"

Figure 6

The superoxide dismutase activity of Arabidopsis thaliana with different periods of time of UV-B radiation CK: Control; B1-B6 see Figure 1"

Figure 7

The catalase activity of Arabidopsis thaliana with different periods of time of UV-B radiation CK: Control; B1-B6 see Figure 1"

Figure 8

The chlorophyll fluorescence characteristics of Arabidopsis thaliana with different periods of time of UV-B radiation CK: Control; B1-B6 see Figure 1"

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