Chin Bull Bot ›› 2013, Vol. 48 ›› Issue (6): 643-650.doi: 10.3724/SP.J.1259.2013.00643

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Cryo-sectioning Conditions and Histochemistry Comparison with Paraffin Sectioning

Jianxia Li1, Chulan Zhang1, Xiaofei Xia2, Liangcheng Zhao1*   

  1. 1College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China

    2Beijing Museum of Nature History, Beijing 100050, China
  • Received:2012-11-01 Revised:2013-01-09 Online:2013-12-03 Published:2013-11-01
  • Contact: Liangcheng Zhao E-mail:zliangcheng@aliyun.com.cn

Abstract: Cryo-sectioning is an important experimental method in plant histology. The freezing temperature and time are the key factors for section quality. In this paper, we compared 15 different conditions and found the optimal freezing temperature and time for direct cryo-sectioning of fresh plant tissues. Also, by observing histochemical staining in 5 different tissues, we compared the similarities and differences between cryo-sectioning and paraffin sectioning in identifying plant chemical substances, and their own application characteristics in plant histochemistry. The 2 methods did not differ in the identification of polysaccharide, protein and cutin, but only cryo-sectioning could be used for fat identification. This study is of value for improving experimental methods in plant histology.

[1]陈丹,赵洁 .适合于植物花器官的冰冻切片技术.[J].武汉植物学研究,2005,23:285-290
[2]刘剑锋,程云清,阎秀峰,周革,王淑范.植物冰冻切片技术的改进[J].南京林业大学学报自然科学版,2006,30:128-130
[3]刘剑锋,阎秀峰,程云清,李霞.冰冻切片技术在高山红景天细胞学研究中的应用[J].东北林业大学学报,2006,34:110,119-110,119
[4]李和平 (2009).植物显微技术第2版.北京:科学出版社,pp.50-50
[5]陆叶,席梦利,郑佳,施季森.杨树和杉木茎段组织的冰冻切片技术研究[J].南京林业大学学报自然科学版,2009,33:44-48
[6]木月惠,李寒冰,贺新强.高度木质化材料的冰冻切片技术[J].植物学通报,2001,18:118-120
[7]宁代锋,尹增芳,张菁,孙海燕.一种简单快速植物组织冰冻切片方法. [J].热带亚热带植物学报,2008,16:386-389
[8]万怡震,贺普超 .葡萄浆果冰冻切片技术参数的研究[J].西北植物学报,2001,21:382-386
[9]谢佩松,徐中根,韦存虚.冰冻切片技术在植物显微结构和组织化学中的应用[J].生物学杂志,2009,26:72-74
[10]张新成,李志刚,李素丽,等.冰冻切片法在植物微管骨架研究中的应用[J].广西植物,2008,28:164-166
[11]Jetter R,Kunst L,Samuels AL (2006).Composition of plant cuticular waxes. In: Riederer M,Müller C (eds). Biology of the Plant Cuticle. Oxford: Blackwell Publishing,pp.182~215
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