Chin Bull Bot ›› 2012, Vol. 47 ›› Issue (6): 625-636.doi: 10.3724/SP.J.1259.2012.00625

Previous Articles     Next Articles

Effect of Location, Harvest Season and Plant Age on Chemical Composition and Antibacterial Activity of Essential Oils from Rosmarinus officinalis

Yan Pan1,2, Hongtong Bai1, Hui Li1, Chuangdao Jiang1, Lei Shi1*   

  1. 1Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

    2Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2012-01-10 Revised:2012-02-27 Online:2012-09-04 Published:2012-11-01
  • Contact: Lei Shi E-mail:shilei67@263.net

Abstract: We examined the effect of plant part, cultivating location, harvest season and plant age on the oil yield, chemical composition and antibacterial activity of essential oils from rosemary (Rosmarinus officinalis). We analyzed essential oils by gas chromatography-mass spectrometry and compared the antibacterial activity against Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Proteus vulgaris. All oil samples showed antibacterial ability against the 4 strains. For Guizhou rosemary, the major compounds of leaf oils were α-pinene (31.88%–39.09%), 1,8-cineole (8.82%–10.97%) and camphor (7.63%–11.59%), whereas those of stem oils were borneol (26.62%–39.05%), camphor (11.41%–15.27%) and α-pinene (7.23%–17.80%). Antibacterial activity was greater for oils from stems than leaves. For oils from Beijing rosemary, the major compounds of leaf oils were camphor (27.92%) and L-β-pinene (25.28%), and the oils showed higher antibacterial activity than those from Guizhou, whereas oils from Beijing plant stems were similar to those from Guizhou plant stems. Oil yields were higher and antibacterial activity stronger with harvesting in June than September and December. In terms of plant age, oil yield, chemical composition and antibacterial activity showed slight differences. Rosemary could be cultivated in parks of Beijing as an ornamental plant and in mountain areas of Guizhou for economic use. Rosemary might be harvested twice in summer and winter, with autumn the second vegetative growing period. Moreover, both leaf and stem parts of rosemary can be used for at least 10 years with little degradation. Our study provides a theoretical basis for maximizing the use of rosemary.

CLC Number: 

  • Q946.8植物次生物质

古昆,程伟贤,李云川,李聪(2003). 云南玉溪产迷迭香挥发油成分分析. 云南大学学报(自然科学版)25, 258-260.
刘兴宽,郁建平,连宾,吴庆松,王德义,时磊(2002). 贵州引种的迷迭香(Rosmarinus officinalis L.)中挥发油化学成分分析. 贵州大学学报(农业与生物科学版) 21, 186-190.
许鹏翔,贾卫民,毕良武,刘先章,赵玉芬(2003). 不同产地的迷迭香精油成分分析及品质研究. 分析科学学报 19, 361-363.
王文中,王颖(1998). 迷迭香天然高效抗氧化剂的研究及其应用. 中国食品添加剂, 26-28.
张冲,李嘉诚,周雪晴,冯玉红,张亚男,张德拉(2008). 超临界CO2萃取迷迭香精油及其化学成分分析. 精细化工 25, 62-67.
中国气象科学数据共享服务网(2010). 中国地面国际交换站气候资料月值数据集. http://cdc.cma.gov.cn/index.html.
周永红,纪良霞(2004). 广西产迷迭香挥发油化学成分的分析. 林产化工通讯 38, 34-36.
Bruneton J (1999). Pharmacognosy, Phytochemistry, Medicinal Plants, 2nd ed. London: Intercept Ltd. pp. 539–540.
Belletti N, Kamdem SS, Tabanelli G, Lanciotti R, Gardini F (2010). Modeling of combined effects of citral, linalool and beta-pinene used against Saccharomyces cerevisiae in citrus-based beverages subjected to a mild heat treatment. Int J Food Microbiol 136, 283-289.
Bounatirou S, Smiti S, Miguel MG, Faleiro L, Rejeb MN, Neffati M, Costa MM, Figueiredoe AC, Barroso JG, Pedro LG (2007). Chemical composition, antioxidant and antibacterial activities of the essential oils isolated from Tunisian Thymus capitatus Hoff. et Link. Food Chem 105, 146-155.
Bozin B, Mimica-Dukic N, Samojlik I, Jovin E (2007). Antimicrobial and antioxidant properties of rosemary and sage (Rosmarinus officinalis L. and Salvia officinalis L., Lamiaceae) essential oils J Agric Food Chem 55, 7879-7885.
Cadun A, Kisla D, Cakli S (2008). Marination of deep-water pink shrimp with rosemary extract and the determination of its shelf-life. Food Chem 109, 81-87.
Celiktas OY, Kocabas EEH, Bedir E, Sukan FV, Ozek T, Baser KHC (2007). Antimicrobial activities of methanol extracts and essential oils of Rosmarinus officinalis, depending on location and seasonal variations. Food Chem 100, 553-559.
Cioni PL, Flamini G, Castellini CB, Ceccarini L, Macchia M (2006). Composition and yield of the essential oils from whole plant, leaves and branches of Rosmarinus officinalis L. growing in minor islands of "Parco Nazionale dell'Arcipelago Toscano". Proceedings of the 1st International Symposium on the Labiatae: Advances in Production, Biotechnology and Utilisation, 255-260
476.
Flamini G, Cioni PL, Morelli I, Macchia M, Ceccarini L (2002). Main agronomic-productive characteristics of two ecotypes of Rosmarinus officinalis L. and chemical composition of their essential oils. J Agr Food Chem 50, 3512-3517.
Gachkar L, Yadegari D, Rezael MB, Taghizadeh M, Astaneh SA, Rasooli I (2007). Chemical and biological characteristics of Cuminum cyminum and Rosmarinus officinalis essential oils. Food Chem 102, 898-904.
Herms DA, Mattson WJ (1992). The Dilemma of Plants - to Grow or Defend. Q Rev Biol 67, 283-335.
Hidalgo PJ, Ubera JL, Tena MT, Valcarcel M (1998). Determination of the carnosic acid content in wild and cultivated Rosmarinus officinalis. J Agr Food Chem 46, 2624-2627.
Klancnik A, Guzej B, Kolar MH, Abramovic H, Mozina SS (2009). In vitro antimicrobial and antioxidant activity of commercial rosemary extract formulations. J Food Prot 72, 1744-1752.
Luqman S, Dwivedi GR, Darokar MP, Kalra A, Khanuja SP (2007). Potential of rosemary oil to be used in drug-resistant infections. Altern Ther Health Med 13, 54-59.
Miguel MG, Guerrero C, Rodrigues H, Brito J, Duarte F, Venancio F, Tavares R (2007). Essential oils of Rosmarinus officinalis L., effect of harvesting dates, growing media and fertilizers. EEESD '07: Proceedings of the 3rd IASME/WSEAS International Conference on Energy, Environment, Ecosystems and Sustainable Development, 65-70
630.
Okoh OO, Sadimenko AP, Afolayan AJ (2010). Comparative evaluation of the antibacterial activities of the essential oils of Rosmarinus officinalis L. obtained by hydrodistillation and solvent free microwave extraction methods. Food Chem 120, 308-312.
Ozcan MM, Chalchat JC (2008). Chemical composition and antifungal activity of rosemary (Rosmarinus officinalis L.) oil from Turkey. Int J Food Sci Nutr 59, 691-698.
Papageorgiou V, Gardeli C, Mallouchos A, Papaioannou M, Komaitis M (2008). Variation of the chemical profile and antioxidant behavior of Rosmarinus officinalis L. and Salvia fruticosa Miller grown in Greece. J Agr Food Chem 56, 7254-7264.
Pintore G, Usai M, Bradesi P, Juliano C, Boatto G, Tomi F, Chessa M, Cerri R, Casanova J (2002). Chemical composition and antimicrobial activity of Rosmarinus officinalis L. oils from Sardinia and Corsica. Flavour Frag J 17, 15-19.
Presti ML, Ragusa S, Trozzi A, Dugo P, Visinoni F, Fazio A, Dugo G, Mondello L (2005). A comparison between different techniques for the isolation of rosemary essential oil. J Sep Sci 28, 273-280.
Rossello JA, Cosin R, Boscaiu M, Vicente O, Martinez I, Soriano P (2006). Intragenomic diversity and phylogenetic systematics of wild rosemaries (Rosmarinus officinalis L. s.l., Lamiaceae) assessed by nuclear ribosomal DNA sequences (ITS). Plant Syst Evol 262, 1-12.
Santoyo S, Cavero S, Jaime L, Ibanez E, Senorans FJ, Reglero G (2005). Chemical composition and antimicrobial activity of Rosmarinus officinalis l. essential oil obtained via supercritical fluid extraction. J Food Protect 68, 790-795.
Serrano E, Palma J, Tinoco T, Venancio F, Martins A (2002). Evaluation of the essential oils of rosemary (Rosmarinus officinalis L.) from different zones of "Alentejo" (Portugal). J Essent Oil Res 14, 87-92.
Singh M (2004). Effects of plant spacing, fertilizer, modified urea material and irrigation regime on herbage, oil yield and oil quality of rosemary in semi-arid tropical conditions. J Hortic Sci Biotech 79, 411-415.
Takaki I, Bersani-Amado LE, Vendruscolo A, Sartoretto SM, Diniz SP, Bersani-Amado CA, Cuman RKN (2008). Anti-Inflammatory and Antinociceptive Effects of Rosmarinus officinalis L. Essential Oil in Experimental Animal Models. Journal of Medicinal Food 11, 741-746.
Tigrine-Kordjani N, Chemat F, Meklati BY, Tuduri L, Giraudel JL, Montury M (2007). Relative characterization of rosemary samples according to their geographical origins using microwave-accelerated distillation, solid-phase microextraction and Kohonen self-organizing maps. Anal Bioanal Chem 389, 631-641.
Zaouali Y, Bouzaine T, Boussaid M (2010). Essential oils composition in two Rosmarinus officinalis L. varieties and incidence for antimicrobial and antioxidant activities. Food and Chemical Toxicology 48, 3144-3152.
No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Yu Feng-lan;Wang Jing-ping;Li Jing-min and Shan Xue-qin. The Isolation and Identification of Sterols and Other Constituents from Seed Fat of Sapium sebiferum[J]. Chin Bull Bot, 1989, 6(02): 121 -123 .
[2] LI Al-Fen;CHEN Min amd ZHOU Bai-Cheng. Advances and Problems in Studies of Photosynthetic Pigment-Protein Complexes of Brown Algae[J]. Chin Bull Bot, 1999, 16(04): 365 -371 .
[3] CHEN Xiao-Mei and GUO Shun-Xing. Research Advances in Plant Disease Resistive Material[J]. Chin Bull Bot, 1999, 16(06): 658 -664 .
[4] LI Ji-Quan JIN You-Ju SHEN Ying-Bai HONG Rong. The Effect of Environmental Factors on Emission of Volatile Organic Compounds from Plants[J]. Chin Bull Bot, 2001, 18(06): 649 -656 .
[5] . [J]. Chin Bull Bot, 2005, 22(增刊): 157 .
[6] Jianxia Li, Chulan Zhang, Xiaofei Xia, Liangcheng Zhao. Cryo-sectioning Conditions and Histochemistry Comparison with Paraffin Sectioning[J]. Chin Bull Bot, 2013, 48(6): 643 -650 .
[7] JIANG Yang-Ming, CUI Wei-Hong, and DONG Qian-Lin. Comprehensive evaluation and analysis of tobacco planting environment based on space technology[J]. Chin J Plan Ecolo, 2012, 36(1): 47 -54 .
[8] Hu Cheng-biao, Zhu Hong-guang, Wei Yuan-lian. A Study on Microorganism and Biochemical Activity of Chinese-fir Plantation on Different Ecological Area in Guangxi[J]. Chin J Plan Ecolo, 1991, 15(4): 303 -311 .
[9] Hong-Xin SU Fan BAI Guang-Qi LI. Seasonal dynamics in leaf area index in three typical temperate montane forests of China: a comparison of multi-observation methods[J]. Chin J Plan Ecolo, 2012, 36(3): 231 -242 .
[10] AN Ran, GONG Ji-Rui, YOU Xin, GE Zhi-Wei, DUAN Qing-Wei, YAN Xin. Seasonal dynamics of soil microorganisms and soil nutrients in fast-growing Populus plantation forests of different ages in Yili, Xinjiang, China[J]. Chin J Plan Ecolo, 2011, 35(4): 389 -401 .