Chin Bull Bot ›› 2012, Vol. 47 ›› Issue (4): 413-421.doi: 10.3724/SP.J.1259.2012.00413

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Genetic Diversity in Natural Populations of Abies chensiensis Based on Nuclear Simple Sequence Repeat Markers

Weimin Li, Sifeng Li*, Bin Li   

  1. Xi’an Botanical Garden of Shaanxi Province, Institute of Botany of Shaanxi Province, Xi’an 710061, China
  • Received:2012-01-29 Revised:2012-04-13 Online:2012-07-26 Published:2012-07-01
  • Contact: Sifeng Li

Abstract: We analyzed the genetic diversity of 6 natural populations of Abies chensiensis (120 individuals) based on 10 simple sequence repeat markers to clarify genetic diversity, genetic structure and changes in gene flow in the populations. We detected 149 alleles in 10 microsatellite loci; the mean number of alleles per locus (A) was 14.9; the effective number of alleles per locus (Ne) was 7.7; the mean expected heterozygosity (He) was 0.841; and the mean observed heterozygosities per locus (Ho) was 0.243. The Shannon diversity index (I) was 2.13, with the proportion of genetic differentiation among populations (FST) 6.7%. Gene flow between the populations (Nm) was 3.45. We found no significant correlation between genetic distance and geographic distance (r=0.490 6, P>0.05). Genetic diversity in the natural population of A. chensiensis was relatively low, with genetic variation mainly among populations. Our results, a field survey and related literature suggest that the gene flow between natural populations in A. chensiensis is higher than before, with perhaps distant hybridization between populations.

陈小勇 (2000). 生境片断化对植物种群遗传结构的影响及植物遗传多样性保护. 生态学报
20, 885–892.
陈小勇 (1996). 植物的基因流及其在濒危植物保护中的作用. 生物多样性 4, 97–102.
Chunlan L, Susumu G, Taizo H (2007). Microsatellite markers for Sachalin fir (Abies sachalinens-is Masters). Molecular Ecology Notes 7, 896–898.
Cremer E, Liepelt S, Sebastiani F, Buonamici A, Michalczyk IM, Ziegenhagen B, Vendrami
GG (2006). Identification and characterization of nuclear microsatellite loci in Abies alba
Mill. Molecular Ecology Notes 6, 374–376.
Doyle JJ, Doyle JE (1987). A rapid DNA isolation procedure for small quantities of fresh
leaf tissue. Phytochemistry Bulletin19, 11–15.
El-Kassaby YA, Sziklai O (1982). Genetic variation of allozyme and quantitative traits in a
selected Douglas-fir Population. Forest Ecology Management 4, 115–126.
El-Kassaby YA (1991). Genetic variation within and among conifer population: Review and
evaluation of methods. In: Finesehi S, Maluolti ME, Cannata F, eds. Biochemical Markers
in the Population Genetics of Frest trees.The Netherlands: Academic Publishing. pp. 61–
葛颂, 洪德元 (1994). 遗传多样性及其检测方法. 见: 钱迎倩, 马克平主编. 生物多样性研
究的原理和方法. 北京: 中国科学技术出版社.
葛颂, 王明麻, 陈岳武 (1988). 用同工酶研究马尾松群体的遗传结构. 林业科学. 24, 399–
葛颂, 王海群, 张灿明, 洪德元 (1997). 八面山银杉林的遗传多样性和群体分化. 植物学报,
39, 266–271.
Hamrick JL, Godt MJ (1989). Allozyme diversity in plant speeies. In: Brown AHD, Clegg
MT, Kahler A L, Weir BS, eds. Plant Population genetics. breeding and genetic resources,
Sunderland. pp. 43-63.
Hamrick JL, Godt MJW, Sherman-Broyles SL (1995) Gene flow among Plant Population:
evidence from genetic makers. In: Hoch PC and AG Stephenson(eds), Experimental and
Molecular approaches to Plant biosystematics. Missouri Botanical Garden press. pp. 215
Hansen OK, Vendramin GG, Sebastiani F, Edwardss J (2005) Development of microsate-
llite markers in Abies nordmanniana (Stev.) Spach and cross-species amplification in the Abies genus. Molecular Ecology Notes 5, 784–787.
赖江山, 李庆梅, 谢宗强 (2003). 濒危植物秦岭冷杉种子萌发特性的研究. 植物生态学报,
27, 661–666.
Linhart YB, Mitton JB, Sturgeon KB, Davis ML (1981). Genetic variation in space and time
in a Population of Ponderosa Pine. Heredity 46, 407–426.
Slatkin M (1985). Rare alleles as indicators of gene flow. Evolution 39, 53–65.
Slatkin M (1985). Gene flow in natural Populations. Ann. Rev. Ecol. Syst 16, 393–430.
孙玉玲, 李庆梅, 谢宗强 (2005). 濒危植物秦岭冷杉结实特性的研究. 植物生态学报, 29, 251–257.
Wang YH, Luo JX, Xue XM, Korpelainen H, Li CY (2005). Diversity of microsatellite
markers in the populations of Picea asperata originating from the mountains of China.
Plant Science 168, 707–714.
Wright S (1931). Evolution in Mendelian Populations. Genetics 16, 97–159.
许晓波 (2005). 濒危植物秦岭冷杉种群动态研究. 硕士论文. 陕西: 西北农林科技大学.
张文辉, 许晓波, 周建云, 孙玉玲, 谢宗强 (2004). 濒危植物秦岭冷杉地理分布和生物生态
学特性研究. 生物多样性, 12 , 419–426.
张文辉, 许晓波, 周建云 (2006). 濒危植物秦岭冷杉生殖生态学特征. 生态学报, 26, 2418
张文辉, 许晓波, 周建云, 谢宗强 (2005). 濒危植物秦岭冷杉种群数量动态. 应用生态学报,
16, 1799–1804.
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