Chin Bull Bot ›› 2012, Vol. 47 ›› Issue (2): 162-170.doi: 10.3724/SP.J.1259.2012.00162

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Establishment of Sesame Callus Induction and Shoot Regeneration System

Hongmei Miao, Ming Ju, Libin Wei, Qin Ma, Haiyang Zhang*   

  1. Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
  • Received:2011-09-29 Revised:2011-12-30 Online:2012-03-16 Published:2012-03-01
  • Contact: Haiyang Zhang E-mail:zhy@hnagri.org.cn

Abstract: We examined the effect of genotypes, explant types, combinations and concentrations of growth regulators on sesame callus induction and shoot regeneration with the cultivars Sesamum indicum L., 2n=26; the wild species S. radiatum, 2n=64, and S. schinzianum, 2n=64; and their distant hybrid strain S. schinzianum × S. indicum. We established the technical system of sesame callus induction and shoot regeneration with high frequency. Among the growth regulators, BA and NAA are suitable for inducing compact green callus, which gives a high differentiation frequency. The optimal medium for sesame callus induction and differentiation was MS basal medium supplemented with 0.1 mg·L–1 NAA, 2.0 mg·L–1 6-BA and 30 g·L–1 sucrose. With this medium, the ratios of the highest level of callus induction and differentiation were 97.50% and 94.02% respectively, which were obtained from wild sesame hypocotyl explants, with the corresponding frequencies of 40.60% and 8.16% for cultivar hypocotyl explants. For the immature embryo explants from the distant hybrids, we achieved the maximum ratios of callus induction (46.67%) and differentiation (89.29%) with this medium. These results form the basis for both germplasm conservation and transgene studies in sesame.

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