Chin Bull Bot ›› 2012, Vol. 47 ›› Issue (2): 162-170.doi: 10.3724/SP.J.1259.2012.00162
Hongmei Miao, Ming Ju, Libin Wei, Qin Ma, Haiyang Zhang*
We examined the effect of genotypes, explant types, combinations and concentrations of growth regulators on sesame callus induction and shoot regeneration with the cultivars Sesamum indicum L., 2n=26; the wild species S. radiatum, 2n=64, and S. schinzianum, 2n=64; and their distant hybrid strain S. schinzianum × S. indicum. We established the technical system of sesame callus induction and shoot regeneration with high frequency. Among the growth regulators, BA and NAA are suitable for inducing compact green callus, which gives a high differentiation frequency. The optimal medium for sesame callus induction and differentiation was MS basal medium supplemented with 0.1 mg·L–1 NAA, 2.0 mg·L–1 6-BA and 30 g·L–1 sucrose. With this medium, the ratios of the highest level of callus induction and differentiation were 97.50% and 94.02% respectively, which were obtained from wild sesame hypocotyl explants, with the corresponding frequencies of 40.60% and 8.16% for cultivar hypocotyl explants. For the immature embryo explants from the distant hybrids, we achieved the maximum ratios of callus induction (46.67%) and differentiation (89.29%) with this medium. These results form the basis for both germplasm conservation and transgene studies in sesame.
Hongmei Miao, Ming Ju, Libin Wei, Qin Ma, Haiyang Zhang. Establishment of Sesame Callus Induction and Shoot Regeneration System[J].Chin Bull Bot, 2012, 47(2): 162-170.
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