Chin Bull Bot ›› 2011, Vol. 46 ›› Issue (6): 688-693.doi: 10.3724/SP.J.1259.2011.00688

Previous Articles     Next Articles

Use of Matrix Attachment Region in Regulating Transgene Expression of Tomato

Aiqing Sun1, Shujuan Ge2, Jiedao Zhang2*   

  1. 1College of Agronomy, Shandong Agricultural University, Taian 271018, China;
    2 Shandong Provincial Key Laboratory ofCrop Biology, State Key Laboratory of Crop Science, College of Life Sciences, Shandong Agricultural University, Taian 271018, China
  • Received:2011-06-21 Revised:2011-09-03 Online:2011-11-18 Published:2011-11-01
  • Contact: Jiedao Zhang

Abstract: Use of matrix attachment regions (MARs) is an important method to improve gene transformation and transgenic expression in plants. We constructed the TM2 MAR from tobacco at the flanking sides of the expression cassettes of reporter gene GUSA and selective gene NPTII in a plant expression vector, pBI121. The recombinant constructs were introduced into tomato plants by Agrobacterium-mediated cotyledon transformation. The transgenes were increasingly transformed and expressed in the transformants. Comparison of the effect of TM2 on different lengths of CaMV 35S promoter showed that the transcription enhancement of TM2 depended on the existence of promoter and showed partial functional redundancy. The effect of TM2 on transcription regulated by heat-inducible promoter indicated that the MAR did not change the regulation pattern but improved the expression level of the heat-inducible promoter. These characteristics of TM2 MAR fulfill the regulation requirements for transgenic expression and can be widely used in diverse plant biotechnology.

[1] McCormick S, NiedermeyerJ, Fry J, Barnason A, Horsch R, Fraley R(1986) Leaf disc transformation of cultivated tomato(L. esculentum) using Agrobacterium tumeficiens. Plant Cell Rep 5,81-84.
[2] Wroblewski T, Tomczak A, Michelmore R (2005) Optimization of Agrobacterium-mediated transient assays of gene expression in lettuce, tomato and Arabidopsis.Plant Biotechnol J 3,259-273.
[3] Orzaez D, Mirabel S, Wieland WH, Granell A (2006) Agroinjection of tomato fruits. A tool for rapid functional analysis of transgenes directly in fruit. Plant Physiol 140,3-11.
[4] Pozueta-Romero J, Houlné G, Ca?as L, Schantz R, Chamarro J (2001)Enhanced regeneration of tomato and pepper seedling explants for Agrobacterium-mediated transformation. Plant Cel lTiss Org Cult 67,173-180.
[5] Park SH, Morris JL, Park JE, Hirschi KD, Smith RH(2003) Efficient and genotype-independent Agrobacterium– mediated tomato. J Plant Physiol 160,1253-1257
[6]Velcheva M, Faltin Z, Flaishman M,Eshdat Y,Perl A.(2005) A liquid culture system for Agrobacterium-mediated transformation of tomato(Lycopersicon esculentum L. Mill). Plant Sci 168,121-130.
[7]Frary A, Earle ED (1996) An examination of factors affecting the efficient of Agrobacterium-mediated transformation of tomato. Plant Cell Rep 16,235-240.
[8]Sun HJ, Uchii S, Watanabe S, Ezura H(2006) A highly efficient transformation protocol for Micro-Tom, a model cultivar for tomato functional genomics.Plant Physiol 47,426-431.
[9] De Amicis F, Patti T, Marchetti S(2007) Improvement of the pBI121 plant expression vector by leader replacement with a sequence combining a poly(CAA) and a CT motif. Transgenic Res 16,731-738
[10] Kay R, Chan A, Daly M, McPherson J(1987) Duplication of CaMV 35S Promoter Sequences Creates a Strong Enhancer for Plant Genes.Science 236,1299-302.
[11] Avramova Z, Sanmiguel P, Georgieva E, Bennetzen JL(1995) Matrix attachment regions and transcribed sequences within a long chromosomal continuum containing maize Adh1. Plant Cell 7,1667-1680.
[12] Fukuda Y (1999) Characterization of matrix attachment sites in the upstream region of a tobacco chitinase gene. Plant Mol Biol 39,1051-1062.
[13] Hall G Jr, Allen GC, Loer DS, Thompson WF, Spiker S(1991) Nuclear scaffold and scaffold-attachment regions in higher plants. Proc Natl Acad Sci USA 88,9320-9324.
[14] Xue H, Yang Y T, WuCA, Ya ng G.D, Zhang MM, Zheng CC(2005) TM2, a novel strong matrix attachment region isolated from tobacco, increases transgene expression in transgenic rice calli and plants. Theor Appl Genet 110,620-627.
[15] Zhang JD, Lu L, Ji LS, Yang GD, Zheng CC(2009) Functional characterization of a tobacco matrix attachment region-mediated enhancement of transgene expression. Transgenic Res 18,377-85.
No related articles found!
Full text



[1] Zhang Zhen-jue. Some Principles Governing Shedding of Flowers and Fruits in Vanilla fragrans[J]. Chin Bull Bot, 1985, 3(05): 36 -37 .
[2] Qian Gao;Yuying Liu;Yinan Fei;Dapeng Li;Xianglin Liu* . Research Advances into the Root Radial Patterning Gene SHORT-ROOT[J]. Chin Bull Bot, 2008, 25(03): 363 -372 .
[3] Wang Bao-shan;Zou Qi and Zhao Ke-fu. Advances in Mechanism of Crop Salt Tolerance and Strategies for Raising Crop Salt Tolerance[J]. Chin Bull Bot, 1997, 14(增刊): 25 -30 .
[4] HE Feng WU Zhen-Bin. Application of Aquatic Plants in Sewage Treatment and Water Quality Improvement[J]. Chin Bull Bot, 2003, 20(06): 641 -647 .
[5] TIAN Bao-Lin WANG Shi-Jun LI Cheng-Sen CHEN Gui-Ren. An Approach on the Origin Center, Evolution Center and the Mechanics of Evolution and Extinction of the Late Palaeozoic Cathaysian Flora[J]. Chin Bull Bot, 2000, 17(专辑): 21 -33 .
[6] ZHANG Yan FANG Li LI Tian-Fei YAO Zhao-BingJIANG Jin-Hui. Effect of Calcium on the Heat Tolerance and Active Oxygen Metabolism of Tobacco Leaves[J]. Chin Bull Bot, 2002, 19(06): 721 -726 .
[7] JIA Hu-Sen LI De-QuanHAN Ya-Qin. Cytochrome b-559 in Chloroplasts[J]. Chin Bull Bot, 2001, 18(02): 158 -162 .
[8] Wei Sun;Chonghui Li;Liangsheng Wang;Silan Dai*. Analysis of Anthocyanins and Flavones in Different-colored Flowers of Chrysanthemum[J]. Chin Bull Bot, 2010, 45(03): 327 -336 .
[9] Dapeng Li;Min Zhang;Qian Gao;Yong Hu;Yikun He*. An Emerging Picture of Plastid Division in Higher Plants[J]. Chin Bull Bot, 2009, 44(01): 43 -51 .
[10] . Phosphate_Stress Protein and Iron_Stress Protein in Plants[J]. Chin Bull Bot, 2001, 18(05): 571 -576 .