Chinese Bulletin of Botany ›› 2011, Vol. 46 ›› Issue (5): 569-574.doi: 10.3724/SP.J.1259.2011.00569

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Callus Induction and Plant Regeneration of Rose

Xue Chen, Jinzhu Zhang, Bingbing Pan, Chengjin Sang, Xue Ma, Tao Yang, Daidi Che*   

  1. Horticulture College of Northeast Agricultural University, Harbin 150030, China
  • Received:2011-05-09 Revised:2011-07-05 Online:2011-09-01 Published:2011-09-01
  • Contact: Daidi Che E-mail:daidiche@yahoo.com.cn

Abstract: We used the leaves and stems of aseptic seedlings of 5 rose cultivars that can successfully live outside through the winter in Heilongjiang Province as explants to investigate callus induction and plant regeneration. The leaves and stems of the 5 rose cultivars could induce callus; media containing 2,4-D could induce callus better; and a high concentration of cytokinin was not suitable for callus induction. TDZ played an important role in callus differentiation; culture under light conditions was effective for callus differentiation; and callus differentiation decreased with increasing number of subcultures. Plant regeneration could be successfully induced from the callus of 2004-8 and 2004-9 in experimentation, and the callus differentiation rate of the 2 varieties was 45% and 38%, respectively.

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