Chin Bull Bot ›› 2011, Vol. 46 ›› Issue (3): 302-310.doi: 10.3724/SP.J.1259.2011.00302

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Using Leaf Sections to Identify Different Salt Tolerance Potential of Poplar Plants

Ying Zhang1, Yingxia Yang1, Yanhong Jia2, Xiangming Zhou2, Lili Nie2, Yue Zhang2, Shouyi Chen3, Jingan Wang1*, Zhongqi Liu2*   

  1. 1College of Life Sciences, Tianjin Normal University, Tianjin 300384, China;

    2Tianjin Research Center of AgriculturalBio-technology, Tianjin 300192, China;

    3Institute of Genetics and Developmental Biology,Chinese Academy of Sciences, Beijing 100101, China
  • Received:2010-10-08 Revised:2011-01-21 Online:2011-05-26 Published:2011-05-01
  • Contact: Jingan Wang,Zhongqi Liu;

Abstract: Plant dry weight and changes in Na+ and K+ contents in leaves were investigated in 1-year-old cuttings from the receptor poplar 107 (Populus × euramericana ‘Neva’) and transgenic poplar 18-1 after cuttings were cultured in Hoagland solution with different NaCl concentrations. The growth and rooted percentage of 18-1 was higher than that of 107 with 0.6%NaCl, and the accumulation of Na+ in 18-1 leaves was about 1.45 times higher than that in 107. Physiological characteristics in leaf sections from 107 and 18-1 under NaCl stress were determined. Leaf sections from 18-1 displayed lower chlorophyll decreasing speed and relative electrical conductivity than that from 107. When sections of young poplar leaves were plated on MS medium with 1.2%NaCl, leaf sections from 107 stopped growing, but those from 18-1 grew continuously and their length increased nearly 50% after 7 days. Thus, salt tolerance between different genotypes can be identified in leaf sections.

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