Chin Bull Bot ›› 2011, Vol. 46 ›› Issue (1): 67-73.doi: 10.3724/SP.J.1259.2011.00067

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Optimization Process and Method of 2-D Electrophoresis for Rice Proteomics

Chengqiang Ding, Dan Ma, Shaohua Wang, Yanfeng Ding*   

  1. College of Agronomy, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2010-09-20 Revised:2010-11-05 Online:2011-01-20 Published:2011-01-01
  • Contact: Yanfeng Ding

Abstract: 2-D electrophoresis is a powerful tool for analyzing protein mixtures. It has been widely used in proteome research. Rice is an important food crop, and proteomic research into rice has been extensive. Because of the technical complexity and time constraints, performing the procedure accurately has been difficult. This paper describes an optimization process for 2-D electrophoresis of various organs of rice. This method can help researchers optimize experimental conditions with better accuracy.

丁坤善, 郑彩霞, 包仁艳, 姜春宁 (2005) 油松雌性不育系球果蛋白质双向电泳. 植物学通报 22, 190-197
付忠军, 丁冬, 进茜宁, 王长城, 李永亮, 汤继华 (2009) 玉米花丝蛋白质组双向电泳条件的优化. 植物生理学通讯 45, 1215-1220
王清, 产祝龙, 秦国政, 田世平 (2009) 果实蛋白质组学研究的实验方法. 植物学报 44, 107-116
Ali GM, Komatsu S (2006) Proteomic analysis of rice leaf sheath during drought stress. Journal of proteome research 5, 396-403
Bokhari SA, Wan XY, Yang YW, Zhou L, Tang WL, Liu JY (2007) Proteomic response of rice seedling leaves to elevated CO2 levels. Journal of proteome research 6, 4624-4633
Ge C, Wan D, Wang Z, Ding Y, Wang Y, Shang Q, Ma F, Luo S (2008) A proteomic analysis of rice seedlings responding to 1,2,4-trichlorobenzene stress. J Environ Sci (China) 20, 309-319
Hashimoto M, Komatsu S (2007) Proteomic analysis of rice seedlings during cold stress. Proteomics 7, 1293-1302
He H, Li J (2008) Proteomic analysis of phosphoproteins regulated by abscisic acid in rice leaves. Biochem Biophys Res Commun 371, 883-888
Jagadish SV, Muthurajan R, Oane R, Wheeler TR, Heuer S, Bennett J, Craufurd PQ (2010) Physiological and proteomic approaches to address heat tolerance during anthesis in rice (Oryza sativa L.). Journal of experimental botany 61, 143-156
Kim ST, Kim SG, Hwang DH, Kang SY, Kim HJ, Lee BH, Lee JJ, Kang KY (2004) Proteomic analysis of pathogen-responsive proteins from rice leaves induced by rice blast fungus, Magnaporthe grisea. Proteomics 4, 3569-3578
Lee DG, Ahsan N, Lee SH, Kang KY, Bahk JD, Lee IJ, Lee BH (2007) A proteomic approach in analyzing heat-responsive proteins in rice leaves. Proteomics 7, 3369-3383
Lin SK, Chang MC, Tsai YG, Lur HS (2005) Proteomic analysis of the expression of proteins related to rice quality during caryopsis development and the effect of high temperature on expression. Proteomics 5, 2140-2156
Lin YZ, Chen HY, Kao R, Chang SP, Chang SJ, Lai EM (2008) Proteomic analysis of rice defense response induced by probenazole. Phytochemistry 69, 715-728
Sengupta S, Majumder AL (2009) Insight into the salt tolerance factors of a wild halophytic rice, Porteresia coarctata: a physiological and proteomic approach. Planta 229, 911-929
Wei Z, Hu W, Lin Q, Cheng X, Tong M, Zhu L, Chen R, He G (2009) Understanding rice plant resistance to the Brown Planthopper (Nilaparvata lugens): a proteomic approach. Proteomics 9, 2798-2808
Yan S, Tang Z, Su W, Sun W (2005) Proteomic analysis of salt stress-responsive proteins in rice root. Proteomics 5, 235-244
Yang P, Li X, Wang X, Chen H, Chen F, Shen S (2007) Proteomic analysis of rice (Oryza sativa) seeds during germination. Proteomics 7, 3358-3368
Yang Q, Wang Y, Zhang J, Shi W, Qian C, Peng X (2007) Identification of aluminum-responsive proteins in rice roots by a proteomic approach: cysteine synthase as a key player in Al response. Proteomics 7, 737-749
Yoshida S, Forno DA, Cock JH, Gomez KA (1976) Laboratory manual for physiological studies of rice, 3 Edition edn. Los Banos: International Rice Research Institute, The Philippines
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[1] Zhu Chen;Liu Fei-yan and Zeng Guang-wen. Effects of 4PU on the Senescence of Detached Radish Cotyledons[J]. Chin Bull Bot, 1997, 14(04): 42 -44 .
[2] Yang Guang-xiao. Effects of EMS on Anther Culture of Rice in Vitro[J]. Chin Bull Bot, 1990, 7(02): 41 -45 .
[3] FU Hong CHI Zhe-Ru① CHANG Jie FU Cheng-Xin. Extraction of Leaf Vein Features Based on Artificial Neural Network — Studies on the Living Plant Identification Ⅰ[J]. Chin Bull Bot, 2004, 21(04): 429 -436 .
[4] Hongyan Li;Qingsong Zheng;Zhaopu Liu*;Qing Li. Effects of Various Concentration of Seawater on the Growth and Physiological Characteristics of Lactuca indica Seedlings[J]. Chin Bull Bot, 2010, 45(01): 73 -78 .
[5] . [J]. Chin Bull Bot, 1994, 11(专辑): 10 .
[6] YANG Jia-Ju YI Tie-Mei ZHAO Cai-yun. Nomenclature and Identification of Gymnosperm Fossil Woods in China[J]. Chin Bull Bot, 2000, 17(专辑): 117 -129 .
[7] Yan Liu, Lijing Xing, Junhua Li, Shaojun Dai. Rice B-box Zinc Finger Protein OsBBX25 is Involved in the Abiotic Response[J]. Chin Bull Bot, 2012, 47(4): 366 -378 .
[8] Qiaoling Zhu, Jiayi Leng, Qingsheng Ye. Photosynthetic Characteristics of Dendrobium williamsonii and D. longicornu[J]. Chin Bull Bot, 2013, 48(2): 151 -159 .
[9] . [J]. Chin J Plan Ecolo, 1963, (1): 110 -130 .
[10] Fan Zheng, Hu Shizhi. Report of the 1st National Scientific and Working Conference on the Classification, Regionalization and Mapping of Vegetation[J]. Chin J Plan Ecolo, 1981, 5(2): 147 -148 .