Chinese Bulletin of Botany ›› 2011, Vol. 46 ›› Issue (1): 67-73.DOI: 10.3724/SP.J.1259.2011.00067

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Optimization Process and Method of 2-D Electrophoresis for Rice Proteomics

Chengqiang Ding, Dan Ma, Shaohua Wang, Yanfeng Ding*   

  1. College of Agronomy, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2010-09-20 Revised:2010-11-05 Online:2011-01-01 Published:2011-01-20
  • Contact: Yanfeng Ding

Abstract: 2-D electrophoresis is a powerful tool for analyzing protein mixtures. It has been widely used in proteome research. Rice is an important food crop, and proteomic research into rice has been extensive. Because of the technical complexity and time constraints, performing the procedure accurately has been difficult. This paper describes an optimization process for 2-D electrophoresis of various organs of rice. This method can help researchers optimize experimental conditions with better accuracy.