Chin Bull Bot ›› 2011, Vol. 46 ›› Issue (1): 28-36.doi: 10.3724/SP.J.1259.2011.00028

Previous Articles     Next Articles

Impact of Drought Stress on the Ultrastructure of Leaf Cells in Three Barley Genotypes Differing in Level of Drought Tolerance

Jianhui Chen1,2, Ronghua Li1,2, Peiguo Guo1,2*, Yanshi Xia1, Changen Tian1,2, Shenyu Miao1,2   

  1. 1Guangzhou Key Laboratory on Functional Studies for Plant Stress-resistant Genes, Guangzhou University, Guangzhou510006, China;

    2College of Life Sciences, Guangzhou University, Guangzhou 510006, China
  • Received:2010-08-24 Revised:2010-10-27 Online:2011-01-20 Published:2011-01-01
  • Contact: Peiguo Guo E-mail:guopg@yahoo.com

Abstract: We examined 3 barley genotypes differing in level of drought tolerance in terms of structural differences at the subcellular level. The subcellular structure did not differ among the 3 genotypes under non-stressed conditions, but nuclei, chloroplasts and mitochondria of leaf cells of all 3 genotypes showed ultra-structural changes after drought stress treatment. In the drought-sensitive genotype Moroc9-75, drought stress caused a high degree of chromatin condensation, altered chloroplast conformation with the waving/swelling of the outer membrane of the chloroplast envelope, and disrupted thylakoids that showed conformational and configurational alterations. In addition, the structure and membrane of the mitochondria was disrupted, and cristae partially disappeared. In the drought-tolerant genotype HS41-1, water shortage caused lighter chromatin condensation than in Moroc9-75, and after drought stress, most chloroplasts and mitochondria in leaf cells showed no significant changes in conformation or configuration. Martin possessed moderate tolerance to drought, and its response to water deficit was between that for HS41-1 and Moroc9-75. Therefore, drought tolerance in barley is related to chloroplast shape, the integrity of grana and stroma thylakoids in chloroplasts and their regular arrangements, the degree of chromatin condensation, and the integrity of mitochondria and cristae. These ultrastructural traits could be used as structural indexes for evaluating drought tolerance in barley.

白志英, 李存东, 屈平 (2009). 干旱胁迫对小麦中国春--Synthetic 6X代换系叶片超微结构的影响. 电子显微学报, 28(1): 68-72
陈珂,焦娟玉,尹春英(2009). 植物对水分胁迫的形态及生理响应.湖北农业科学, 48(4): 992-995
韩善华 (1991). 油菜叶绿体在干旱处理过程中的超微结构变化. 作物学报, 17(4): 311-313
胡化广, 刘建秀,何秋, 郑玉红 (2005). 草坪草种质资源抗旱性及其改良研究进展. 植物学通报, 22(6): 648-657
简令成,王红.逆境植物细胞生物学.北京:科学出版社,2009,115-226
李扬汉. 禾本科作物的形态与解剖.上海:上海科技出版社,1979, 393-416
万里强, 石永红, 李向林, 何峰, 贾亚雄 (2009). 高温干旱胁迫下三个多年生黑麦草品种叶绿体和线粒体超微结构的变化. 草业学报 18(1): 25-31
吴凯,周晓阳 (2007). 环境胁迫对植物超微结构的影响.山东林业科技, 3: 80-83
杨帆,苗灵凤,胥晓,李春阳 (2007). 植物对干旱胁迫的响应研究进展.应用与环境生物学报, 13(4): 586-591
Ceccarelli S (1994). Specific adaptation and breeding for marginal conditions. Euphytica, 77:205-219
Ceccarelli S, Grando S, Baum M, Udupa SM (2004). Breeding for drought resistance in a changing climate. In: Challenges and strategies of dryland agriculture--Rao S, Ryan J, eds. CSSA Special Publication no. 32. Madison, WI: Crop Science Society of America and American Society of Agronomy, 2004, 167-190.
Cellier F, Conejero G, Breitler JC, Casse F (1998). Molecular and physiological responses to water deficit in drought-tolerant and drought-sensitive lines of sunflowers. Accumulation of dehydrin transcripts correlates with tolerance. Plant Physiol, 116:319-328
Cochard H, Coll L, Roux XL, and Améglio T (2002). Unraveling the Effects of Plant Hydraulics on Stomatal Closure during Water Stress in Walnut. Plant Physiol, 128: 282-290
Demirevska K, Simova-Stoilova L, Vassileva V, Feller U (2008). Rubisco and some chaperone protein responses to water stress and real watering at early seedling growth of drought sensitive and tolerant wheat varieties. Plant Growth Regul, 56:97-106
Doorenbos J, Pruit WO (1977). Guidelines for predicting crop water requirements. FAO Irrigation and Drainage Paper no. 24, 1977. Rome: FAO.
Guo P, Baum M, Grando S, Ceccarelli S, Bai G, Li R, von Korff M, Varshney RK, Graner A, Valkoun J (2009). Differentially expressed genes between drought-tolerant and drought-sensitive barley genotypes in response to drought stress during the reproductive stage. J Exp Bot, 60(12): 3531 - 3544.
Guo P, Baum M, Grando S, Ceccarelli S, Li R, Valkoun J (2008). QTLs for chlorophyll and chlorophyll fluorescence parameters in barley under post-flowering drought. Euphytica, 163: 203-214
Gupta S, Berkowitz GA (1988). Chloroplast osmotic adjustment and water stress effects on photosynthesis. Plant Physiol, 88:200–206
Kolodziejek, I, Koziol J, Waleza M, Mostowska A (2003). Ultrastructure of mesophyll cells and pigment content in senescing leaves of maize and barley. Plant Growth Regul, 22: 217-227
Li R, Guo P, Baum M, Grando S and Ceccarelli S (2006). Evaluation of chlorophyll content and fluorescence parameters as indicators of drought tolerance in barley. Agricultural Sciences in China, 5: 751-757
Olmos E, Sánchez-Blanco MJ, Ferrández T, Alarcón JJ (2007). Subcellular effects of drought stress in Rosmarinus officinalis. Plant Biol, 9: 77–84
Radyuk MS and Homan NM (2002). Discrete character of the development of the photosynthetic apparatus in greening barley leaves. Photosynthesis Research, 72: 117-122
Reddy AR , Chaitanya KV , Vivekanandan M (2004). Drought induced response of photosynthesis and antioxidant metabolism in higher plants. Journal of Plant Physiology, 161: 1189-1202
Ristic Z and Cass DD (1991). Chloroplast structure after water shortage and high temperature in two lines of Zea Mays L. that differ in drought resistance. Bot Gaz, 152(2): 186-194.
Ryan J, Estefan G, Rashid A (2001), Soil and plant analysis laboratory manual. Second edition. Jointly published by the Internation Center for Agricultural Research in the Dry Areas (ICARDA) and the National Agricultural Research Center (NARC). Aleppo, Syria.
Sacks MM, Silk WK, Burman P (1997). Effect of water stress on cortical cell division rates within the apical meristem of primary roots of maize. Plant Physiol, 114:519-527
Stoyanova D, Tchakalova E, Yordanov I (2002). Influence of different soil moisture on anatomy of maize leaves and ultrastructure of chloroplasts. Bulg J Plant Physiol, 28(12): 11-20
Vassileva V, Simova-Stoilova L, Demirevska K and Feller U (2009). Variety-specific response of wheat (Triticum aestivum L.) leaf mitochondria to drought stress. Journal of Plant Research, 122(4): 445-454
No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Zhang Zhao-jie and Zheng Yi-jin. The Application of the Structure of Leaf Epidermis in theClassiffication of Ulmus[J]. Chin Bull Bot, 1990, 7(01): 40 -42 .
[2] Zhu Yong-liang. Molecular Aspects of Pollen-Pistil Interaction[J]. Chin Bull Bot, 1988, 5(04): 211 -213 .
[3] Wenyin Zhu;Dewei Yang;Jing Lin;Ling Zhao;Yadong Zhang;Zhen Zhu;Tao Chen;Cailin Wang*. Substitution Mapping of Quantitative Trait Loci for Seed Shattering in Chromosome Segment Substitution Lines of Rice (Oryza sativa L.)[J]. Chin Bull Bot, 2008, 25(04): 443 -448 .
[4] Chen Li;Xiufen Song;Chunming Liu. Peptide Hormones in Higher Plants[J]. Chin Bull Bot, 2006, 23(5): 584 -594 .
[5] DENG Sheng-Hui SHANG Ping. A Brief Review of the Mesozoic Filicopsida in China[J]. Chin Bull Bot, 2000, 17(专辑): 61 -73 .
[6] Caifeng Jia;Yue Li . Somatic Embryogenesis of Immature Embryos in Pinus armandii[J]. Chin Bull Bot, 2006, 23(2): 186 -191 .
[7] Li Xian-zhang and Tang Ding-tai. Effect of the Different Preservative Solutions on the Cut Flower Life and Storage of Tulip[J]. Chin Bull Bot, 1995, 12(增刊): 66 -68 .
[8] XU Zhao-Liang. Progress of Study on the Megaalgal Fossils from the Early Cambrian, Chengjiang[J]. Chin Bull Bot, 2000, 17(专辑): 289 -291 .
[9] Li Yun-ying;Cao Min;Dang Feng-liang;Wang Jian;Zhou Yu-shan and Gao Huan. Effect of Different Storage Temperature on the Occurrence of[J]. Chin Bull Bot, 1984, 2(05): 37 -38 .
[10] Chen Shan-kum Zeng Xiao-chun Liu Zhuan-fei La Mei-hua Wang Xiao-hong Cao Kai-wei. He Effects of PP333 and S-3307 on Reducing Plant Height Promotimg Tillers Growing Vigorous rice seedlings increasing Yield of rice and its relation to phytohormones[J]. Chin Bull Bot, 1995, 12(专辑3): 95 -101 .