Chin Bull Bot ›› 2011, Vol. 46 ›› Issue (1): 21-27.doi: 10.3724/SP.J.1259.2011.00021

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Genetic Analysis of Cold Tolerance at the Seedling Stage in Dongxiang Wild Rice (Oryza rufipogon)

Shuirong Jian1,3, Yong Wan2, Xiangdong Luo1, Jun Fang3, Chengcai Chu3, Jiankun Xie1,2*   

  1. 1College of Life Science, Jiangxi Normal University, Nanchang 330022, China;

    2Rice Research Institute, Jiangxi Academy ofAgricultural Science, Nanchang 300200, China;

    3Institute of Genetics and Developmental Biology, Chinese Academy ofSciences, Beijing 100101, China
  • Received:2010-07-27 Revised:2010-09-24 Online:2011-01-20 Published:2011-01-01
  • Contact: Jiankun Xie E-mail:xiejiankun@yahoo.com

Abstract: We examined the cold tolerance of seedlings of a BILs population (BC1F9) of Dongxiang wild rice (Dwr)/Xieqingzao B (Xqz B)//Xqz B by cold treatment assay and genetic analysis. The control materials included the strong cold-tolerant variety, Longdao 5, and the cold-sensitive cultivar Xqz B, using seedling wilting rate and seedling mortality rate as evaluation indices. After cold treatment (10°C) for 7 days, the mean seedling wilting rate and seedling mortality rate for 228 BILs were 67.4% and 70.8%, respectively. The lines with only 20% seedling mortality rate or below were further treated with a colder temperature of 8°C day/5°C night for 5 days. The lines 5243 and 5335 were the most coldtolerant and may be useful for constructing quantitative trait loci-near-isogenic lines of Dwr. Moreover, the skew-normal and continuous distribution of seedling wilting and mortality rates suggest that cold tolerance at the seedling stage in Dwr represents the inheritance of qualitative-quantitative traits which are controlled by major genes and polygenes.

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