Chin Bull Bot ›› 2017, Vol. 52 ›› Issue (3): 290-296.doi: 10.11983/CBB16097

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Effects of Buffered Cooling in Root Zone on Frost Injury in Grape Leaf

Lulong Sun, Qingwei Geng, Hao Xing, Yuanpeng Du, Heng Zhai*   

  1. College of Horticultural Science and Engineering, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, China
  • Received:2016-04-27 Accepted:2016-05-23 Online:2017-05-27 Published:2017-05-01
  • Contact: Zhai Heng
  • About author:

    # Co-first authors


To study the effects of cooling conditions in the root zone on frost injury in grapevine leaves, we used 1-year-old Merlot grapevine (Vitis vinifera) seedlings. The root zone was cooled regularly or buffered. All seedlings were treated in the simulated frost condition, then the frost index in leaves was calculated, and the chlorophyll fluorescence parameters were analyzed to reflect the change of PSII activity. Root temperature cooled regularly conferred severe frost damage in leaves, with a frost index of 74.36%. Buffered cooling alleviated frost injury to 53.29%, with a frost index of only 21.07%. Buffered cooling in the root zone improved qP and Fvʹ/Fmʹ in leaves during the recovery period, sped up the recovery of photochemical activity in PSII (ΦPSII), improved the ability of heat dissipation (NPQ), and relieved photo- inhibition. Buffered cooling in the root zone was conducive for leaves to recover after frost.

Figure 1

Changes in temperatures of air and soil in the root zone during freezing treatment of grape leavesAir: Temperature in the freezer; Freezing: Roots were frozen during the period of cooling; Buffered cooling: Temperature in the root zone was buffered during the period of cooling"

Table 1

Effects of temperature dropping down conditions in root zone on frost index of grape leaves"

Number of
frozen leaves
Number of
Frost index
Freezing 4.48±2.50 6.04±0.98 74.36±8.13
Buffered 1.56±0.49 7.78±2.01 21.07±6.61**

Figure 2

Effects of delaying root zone temperature dropping down on Fv/Fm (A) and F0 (B) of grape leaves after frost, during the period of recovery (means±SD, n=30)Fv/Fm: Maximum quantum yield of PSII; F0: Dark fluorescence yield"

Figure 3

Effects of delaying root area temperature dropping down on ΦPSII of grape leaves after frost, and during the period of recovery (means±SD, n=30)ΦPSII: Photochemical yield of photosystem II"

Figure 4

Effects of delaying root area temperature dropping down on qP (A) and Fvʹ/Fmʹ (B) of grape leaves after frost, during the period of recovery (means±SD, n=30)qP: Coefficient of photochemical quenching; Fvʹ/Fmʹ: Quantum efficiency of open PSII reaction centers"

Figure 5

Effects of delaying root area temperature dropping down on NPQ of grape leaves after frost, during the period of recovery (means±SD, n=30)NPQ: Non-photochemical quenching"

[1] 郭连旺, 沈允钢 (1996). 高等植物光合机构避免强光破坏的保护机制. 植物生理学通讯 32, 1-8.
[2] 胡文海, 肖宜安, 龙婉婉 (2005). 夜间低温后日间光照对海桐和榕树叶片的光抑制以及光系统II功能的影响. 植物生理学通讯 441, 467-470.
[3] 刘俊, 刘崇怀 (2006). 龙眼葡萄棚架栽培条件下的根系分布. 果树学报 23, 379-383.
[4] 毛娟, 陈佰鸿, 曹建东, 王利军, 王海, 王延秀 (2013). 不同滴灌方式对荒漠区‘赤霞珠’葡萄根系分布的影响. 应用生态学报 24, 3084-3090.
[5] 孙鲁龙, 宋伟, 杜远鹏, 翟衡 (2015). 简易覆盖对泰安地区酿酒葡萄园冬季土壤温湿度的影响. 中外葡萄与葡萄酒 4, 12-16.
[6] Bertamini M, Muthuchelian K, Rubinigg M, Zorer R, Velasco R, Nedunchezhian N (2006). Low-night temperature increased the photo-inhibition of photosynthesis in grapevine (Vitis vinifera L. cv. Riesling) leaves. Environ Exp Bot 57, 25-31.
[7] Demmig-Adams B, Adams III WW, Barker DH (1996). Using chlorophyll fluorescence to assess the fraction of absorbed light allocated to thermal dissipation of excess excitation.Physiol Plant 98, 253-264.
[8] Feng YL, Cao KF, Feng ZL (2002). Thermal dissipation, leaf rolling and inactivation of PSII reaction centers in Amomum villosum in diurnal course. J Tropic Ecol 18, 865-876.
[9] Heber U, Bukhov NG, Shuvalov VA, Kobayashi Y, Lange OL (2001). Protection of the photosynthetic apparatus against damage by excessive illumination in homoiohydric leaves and poikilohydric mosses and lichens.J Exp Bot 52, 1999-2006.
[10] Huang LK, Wong SC, Terashima I, Zhang X, Lin DX, Osmond CB (1989). Chilling injury in mature leaves of rice. I. Varietal differences in the effects of chilling on canopy photosynthesis under simulated ‘dry cold dew wind’ conditions experienced in south-east China.Aust J Plant Physiol 16, 321-337.
[11] Johnson GN, Young AJ, Scholes JD (1993). The dissipation of excess excitation energy in British plant species.Plant Cell Environ 16, 673-679.
[12] Mauro S, Dainese P, Lannoye R, Bassi R (1997). Cold- resistant and cold-sensitive maize lines differ in the phosphorylation of the photosystem II subunit, CP29. Plant Physiol 115, 171-180.
[13] Maxwell K, Johnson GN (2000). Chlorophyll fluorescence—a practical guide.J Exp Bot 51, 659-668.
[14] Molitor D, Caffarra A, Sinigoj P (2014). Late frost damage risk for viticulture under future climate conditions: a case study for the Luxembourgish winegrowing region.Aust J Grape Wine Res 20, 160-168.
[15] Nikiforou C, Nikolopoulos D, Manetas Y (2011). The winter-red-leaf syndrome in Pistacia lentiscus: evidence that the anthocyanic phenotype suffers from nitrogen deficiency, low carboxylation efficiency and high risk of photo- inhibition. J Plant Physiol 168, 2184-2187.
[16] Sonya L (2014). Frost: Canberra sees the silver lining in damaging frosts.Wine Viticult J 29, 48.
[17] Sugiura T, Sumida H, Yokoyama S (2012). Overview of recent effects of global warming on agricultural production in Japan.Jpn Agr Res Q 46, 7-13.
[18] Velikova V, Pinelli P, Loreto F (2005). Consequences of inhibition of isoprene synthesis in Phragmites australis leaves exposed to elevated temperatures. Agr Ecosyst Environ 106, 209-217.
[19] Zhou Y, Huang L, Zhang Y, Shi K, Yu J, Nogués S (2007). Chill-induced decrease in capacity of RuBP carboxylation and associated H2O2 accumulation in cucumber leaves are alleviated by grafting onto figleaf gourd.Ann Bot 100, 839-848.
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[1] Hu Shi-yi. Fertilization in Plants IV. Fertilization Barriers Inoompalibilty[J]. Chin Bull Bot, 1984, 2(23): 93 -99 .
[2] JIANG Gao-Ming. On the Restoration and Management of Degraded Ecosystems: with Special Reference of Protected Areas in the Restoration of Degraded Lands[J]. Chin Bull Bot, 2003, 20(03): 373 -382 .
[3] . [J]. Chin Bull Bot, 1994, 11(专辑): 65 .
[4] . [J]. Chin Bull Bot, 1996, 13(专辑): 103 .
[5] ZHANG Xiao-Ying;YANG Shi-Jie. Plasmodesmata and Intercellular Trafficking of Macromolecules[J]. Chin Bull Bot, 1999, 16(02): 150 -156 .
[6] Chen Zheng. Arabidopsis thaliana as a Model Species for Plant Molecular Biology Studies[J]. Chin Bull Bot, 1994, 11(01): 6 -11 .
[7] . [J]. Chin Bull Bot, 1996, 13(专辑): 13 -16 .
[8] LEI Xiao-Yong HUANG LeiTIAN Mei-ShengHU Xiao-SongDAI Yao-Ren. Isolation and Identification of AOX (Alternative Oxidase) in ‘Royal Gala’ Apple Fruits[J]. Chin Bull Bot, 2002, 19(06): 739 -742 .
[9] Chunpeng Yao;Na Li. Research Advances on Abscisic Acid Receptor[J]. Chin Bull Bot, 2006, 23(6): 718 -724 .
[10] Li Wang, Qinqin Wang, Youqun Wang. Cytochemical Localization of ATPase and Acid Phosphatase in Minor Veins of the Leaf of Vicia faba During Different Developmental Stages[J]. Chin Bull Bot, 2014, 49(1): 78 -86 .