Chin Bull Bot ›› 2017, Vol. 52 ›› Issue (3): 290-296.doi: 10.11983/CBB16097

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Effects of Buffered Cooling in Root Zone on Frost Injury in Grape Leaf

Lulong Sun, Qingwei Geng, Hao Xing, Yuanpeng Du, Heng Zhai*   

  1. College of Horticultural Science and Engineering, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, China
  • Received:2016-04-27 Accepted:2016-05-23 Online:2017-05-27 Published:2017-05-01
  • Contact: Zhai Heng E-mail:zhaih@sdau.edu.cn
  • About author:

    # Co-first authors

Abstract:

To study the effects of cooling conditions in the root zone on frost injury in grapevine leaves, we used 1-year-old Merlot grapevine (Vitis vinifera) seedlings. The root zone was cooled regularly or buffered. All seedlings were treated in the simulated frost condition, then the frost index in leaves was calculated, and the chlorophyll fluorescence parameters were analyzed to reflect the change of PSII activity. Root temperature cooled regularly conferred severe frost damage in leaves, with a frost index of 74.36%. Buffered cooling alleviated frost injury to 53.29%, with a frost index of only 21.07%. Buffered cooling in the root zone improved qP and Fvʹ/Fmʹ in leaves during the recovery period, sped up the recovery of photochemical activity in PSII (ΦPSII), improved the ability of heat dissipation (NPQ), and relieved photo- inhibition. Buffered cooling in the root zone was conducive for leaves to recover after frost.

Figure 1

Changes in temperatures of air and soil in the root zone during freezing treatment of grape leavesAir: Temperature in the freezer; Freezing: Roots were frozen during the period of cooling; Buffered cooling: Temperature in the root zone was buffered during the period of cooling"

Table 1

Effects of temperature dropping down conditions in root zone on frost index of grape leaves"

Number of
frozen leaves
Number of
leaves
Frost index
(%)
Freezing 4.48±2.50 6.04±0.98 74.36±8.13
Buffered 1.56±0.49 7.78±2.01 21.07±6.61**

Figure 2

Effects of delaying root zone temperature dropping down on Fv/Fm (A) and F0 (B) of grape leaves after frost, during the period of recovery (means±SD, n=30)Fv/Fm: Maximum quantum yield of PSII; F0: Dark fluorescence yield"

Figure 3

Effects of delaying root area temperature dropping down on ΦPSII of grape leaves after frost, and during the period of recovery (means±SD, n=30)ΦPSII: Photochemical yield of photosystem II"

Figure 4

Effects of delaying root area temperature dropping down on qP (A) and Fvʹ/Fmʹ (B) of grape leaves after frost, during the period of recovery (means±SD, n=30)qP: Coefficient of photochemical quenching; Fvʹ/Fmʹ: Quantum efficiency of open PSII reaction centers"

Figure 5

Effects of delaying root area temperature dropping down on NPQ of grape leaves after frost, during the period of recovery (means±SD, n=30)NPQ: Non-photochemical quenching"

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