Chin Bull Bot ›› 2017, Vol. 52 ›› Issue (4): 474-479.doi: 10.11983/CBB16052

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Growth Characters, Yield and Quality of Virus-free Rehmannia glutinosa Seedlings in the Field

Xiaoli Zhang1†, Ping Li2†, Caiyun Zhou3, Mingxia Chen1, Xiting Zhao1, Mingjun Li1*   

  1. 1Henan Province Engineering Laboratory of Green Medicinal Plant Biotechnology, Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs, University of Henan Province, College of Life Sciences, Henan Normal University, Xinxiang 453007, China
    2Zhengzhou Shuqing Medical College, Zhengzhou 450064, China
    3Landscape Administrative Office of Luohe City, Luohe 462300, China
  • Received:2016-03-18 Accepted:2016-09-30 Online:2017-05-05 Published:2017-07-01
  • Contact: Li Mingjun E-mail:limingjun2002@263.net
  • About author:

    # Co-first authors

Abstract:

To study the growth, yield and quality improvement of Rehmannia glutinosa virus-free seedlings in the field, we periodically measured the morphology index, physiological characters, photosynthesis index, yield and quality of virus-free seedlings and common seedlings. The virus-free plants were superior to common plants in all growth character indexes. The root yield and quality have been greatly improved. Yield increased by more than 77.35% and catalpol content by 32.90%.

Figure 1

Growth and harvest of virus-free Rehmannia glutinosa seedlings before and after transplanting in field^(A) Virus-free seedlings before transplanting; (B) Virus-free seedlings transplanted to field; (C) Virus-free seedlings growing in field (90 d); (D) Determination of growth characters; (E) Comparison of the root of common seedlings (left) and virus-free seedlings (right) after harvest"

Figure 2

The morphology index of Rehmannia glutinosa virus-free seedlings and common seedlings"

Table 1

The physiological characters of functional leaves of Rehmannia glutinosa virus-free seedlings and common seedlings"

Treatment Content of soluble sugar (mg·g-1) Content of soluble protein
(mg·g-1)
SOD activity
(U·g-1 FW)
POD activity
(U·g-1 FW)
Common seedlings 34.95±3.86 1.67±1.03 110.80±34.08 77.5±25.33
Virus-free seedlings 36.91±2.19 2.06±1.19 109.10±19.31 42.5±18.93

Table 2

The photosynthesis characters of functional leaves of Rehmannia glutinosa virus-free seedlings and common seedlings"

Treatment Content of chlorophyll (mg·g-1) Content of carotenoid
(mg·g-1)
Photosynthetic rate (µmol·m-2·s-1)
Chl a Chl b Chl t
Common seedlings 1.36±0.07 0.45±0.02 1.99±0.11 0.31±0.02 24.12±3.37
Virus-free seedlings 1.48±0.05* 0.49±0.01* 2.16±0.06* 0.36±0.02* 30.62±1.14*

Table 3

The root yield of Rehmannia glutinosa virus-free seedlings and common seedlings"

Location Treatment Density
(Number·hm-2)
Weight of per plant (kg) Output of per hm2
(kg×15)
Yield increase rate (%)
1 Common seedlings 75000 0.468±0.67 2340±336.12 77.35
Virus-free seedlings 0.83±0.16* 4150±777.45*
2 Common seedlings 60000 0.591±0.12 2365.6±485.60 89.55
Virus-free seedlings 1.121±0.29** 4484±1150.49**
3 Common seedlings 90000 0.244±0.77 1463.4±460.18 167.94
Virus-free seedlings 0.654±0.15** 3921±903.11**

Table 4

The root comparation of Rehmannia glutinosa virus-free seedlings and common seedlings"

Treatment Number of root Number of roots over 100 g Number of roots over 200 g Weight of the biggest root (g) Length of the big- gest root (cm) Width of the biggest root (cm)
Common seedlings 12.6±4.7 0.5±0.7 0±0.0 94.81±24.8 15.03±4.6 4.46±0.8
Virus-free seedlings 13.8±4.2 4±1.1** 1.3±1.3** 281.58±116.8** 19.70±3.8* 7.97±2.8**

Table 5

The root quality of Rehmannia glutinosa virus-free seedlings and common seedlings"

Treatment Content of soluble sugar (mg·g-1) Content of soluble protein (mg·g-1) Content of starch
(mg·g-1)
Content of catalpol
(mg·g-1 DW)
Common seedlings 103.44±7.29 1.73±0.07 5.26±0.21 40.79±1.43
Virus-free seedlings 118.09±1.03* 2.43±0.07** 10.11±0.29** 54.21±5.28**
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