Chinese Bulletin of Botany ›› 2016, Vol. 51 ›› Issue (5): 586-593.DOI: 10.11983/CBB15223

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Indole Acetic Acid-Amido Synthetase GH3-6 Negatively Regulates Response to Drought and Salt in Arabidopsis

Xiaodong Liu1, Ruozhong Wang2, Binbin Jiao3, Peihong Dai1, Yue Li1*   

  1. 1College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China
    2Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410128, China
    3Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200135, China
  • Received:2015-12-22 Accepted:2016-04-26 Online:2016-09-01 Published:2016-09-27
  • Contact: Li Yue
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    # Co-first authors

Abstract: Auxin is an important phytohormone involved in almost all aspects of plant life. GH3-6 encodes a protein possessing adenylation activity that can conjugate amino acid to indole acetic acid (IAA), which leads to temporarily or permanently inactive IAA. Here we investigated the role of GH3-6 in plant stress adaptation. The expression of GH3-6 was induced by drought, abscisic acid (ABA) and high salt. Compared with the wild type, transgenic plants with GH3-6 overexpression (named dfl1-D) were sensitive to drought and lost more water from detached rosette leaves. dfl1-D also exhibited hyporesistance to high salt. In addition, overexpression of GH3-6 inhibited the expression of stress-responsive genes, including RD22, KIN1, RD29A and DREB1A. The content of ABA was lower in dfl1-D than the wild type under drought treatment. Overexpression of GH3-6 negatively regulates stress resistance in Arabidopsis.