Chin Bull Bot ›› 2016, Vol. 51 ›› Issue (5): 679-683.doi: 10.11983/CBB15181

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An Optimized Method to Determine Silicon Content in Rice

Yuwei Jia1†, Ruilin Yang2†, Yang Zhang1, Juanjuan Fang1, Hui Chen1*   

  1. 1College of Life Sciences, Shanxi Normal University, Linfen 041004, China
    2Center of Analysis and Test, Shanxi Normal University, Linfen 041004, China
  • Received:2015-10-10 Accepted:2016-02-14 Online:2016-09-27 Published:2016-09-01
  • Contact: Jia Yuwei,Yang Ruilin,Chen Hui E-mail:xhchen_0809@163.com
  • About author:

    # Co-first authors

Abstract:

To establish an easier and more accurate method to determine silicon content in rice, several main parameters of silicon molybdenum-blue spectrophotometry were optimized in combination with 2 sample pretreatments, digestion and autoclaving. By using ascorbic acid as a reductant, with the color development time of the silicon molybdenum yellow and the stable time of silicon molybdenum blue for 5 and 25 min, respectively, optimal silicon content in rice was achieved at 600 nm wavelength by molybdenum-blue spectrophotometry. For sample pretreatment, the digestion method was more effective for silicon extraction than autoclaving. Briefly, the optimized method facilitates the determination of silicon content in rice and other plants and could lay a theoretical foundation for further research of the mechanism of silicon absorbance and transportation.

Figure 1

Optimization of conditions of silicon molybdenum blue spectrophotometric and comparison between two methods of samples pretreatment for determination of silicon content (A) The full wavelength scan figure of three reducing agents (A+B liquid, ascorbic acid, and ammonium iron (II) sulfate); (B) Stable time of silicon molybdenum blue (means±SD, n=9); (C) Silicon standard curve; (D) Comparison of the silicon content in rice samples by using digestion and autoclaving method (means±SD, n=9). Different capital letters represent significant difference across the same samples at different methods (P<0.05, n=9); Different lowercase letters represent significant difference across the same methods at different samples (P<0.05, n=9)."

Table 1

Comparison of measurement using absorbance at the 600 and 812 nm wavelength"

Wavelength (nm) Observation number Minimum OD value Maximum OD value OD value (means±SD)
600 9 0.540 0.557 0.5468±0.006686
812 9 1.621 1.646 1.6302±0.011904

Table 2

Analysis of color development time of silicon molybdenum yellow"

Group Observation number Minimum OD value Maximum OD value OD value (means±SD)
Boiling water bath 30 s 9 0.529 0.532 0.5308±0.0011 c
Standing 5 min 9 0.534 0.536 0.5348±0.0007 a
Standing 10 min 9 0.534 0.536 0.5348±0.0008 a
Standing 15 min 9 0.532 0.535 0.5333±0.0013 ab
Standing 20 min 9 0.528 0.535 0.5321±0.0030 b
Standing 30 min 9 0.528 0.535 0.5320±0.0031 b
1 白淑琴, 阿木日沙那, 那人高娃, 杨帆, 王媛, 王桂花, 横山拓史 (2012). 水稻中硅元素的分布及存在状态. 应用与环境生物学报 18, 444-449.
2 戴伟明, 张克勤, 段彬伍, 孙成效, 郑康乐, 蔡润, 庄杰云 (2005). 测定水稻硅含量的一种简易方法. 中国水稻科学 19, 460-462.
3 石海强, 白淑云, 刘秉钺, 鲁杰 (2011). 稻草制浆中硅含量测定方法的改进. 大连工业大学学报 30, 126-128.
4 童国林, 陆琦, 汪鋆, 周彩虹 (2005). 硅钼蓝光度法测定稻草原料及烧碱法制浆黑液的硅含量. 中华纸业 26, 64-66.
5 王思哲, 温圣贤, 邓文, 蒲熙, 刘灿辉, 崔巍 (2007). 硅肥在水稻上的应用研究进展. 作物研究 21, 620-624.
6 邢雪荣, 张蕾 (1998). 植物的硅素营养研究综述. 植物学通报 15, 33-40.
7 翟庆洲, 金永哲, 邵长路, 张宗韬, 肖丰收, 裘式纶 (1998). 硅钼蓝光度法测定沸石分子筛中的硅. 光谱实验室 15, 82-84.
8 张遴, 蔡砚, 王昌钊, 乐爱山 (2011). 硅钼蓝光度法测定钼铁中硅含量方法的条件优化设计. 化学试剂 33, 829-832.
9 朱智伟, 林榕辉 (1990). 碱氧化消化法快速测定谷壳中的硅. 中国水稻科学 4, 89-91.
10 Liebig JV (刘更另译) (1983). 化学在农业和生理学上的应用(第1版). 北京: 农业出版社. pp. 189-191.
11 Ma JF, Higashitani A, Takeda K (2003). Genotypic variation in silicon concentration of barley grain.Plant Soil 249, 383-387.
12 Ma JF, Mitani N, Nagao S, Konishi S, Tamai K, Lwashita T, Yano M (2004). Characterization of the silicon uptake system and molecular mapping of the silicon transporter gene in rice.Plant Physiol 136, 3284-3289.
13 Meyer ML, Bloom PR (1993). Lithium metaborate fusion for silicon, calcium, magnesium, and potassium analysis of wild rice.Plant Soil 153, 281-285.
14 Okuda A, Takahashi E (1961). Studies on the physiological role of silicon in crop plants. Part1 on the method of silicon-free culture.J Sci Soil Manure Jpn 32, 475-480.
15 Okuda A, Takahashi E (1965). The role of silicon. In: Chandler RF, ed. The Mineral Nutrition of the Rice Plant. Baltimore: John Hopkins Press. pp. 126-146.
16 Yoshida S, Forno DA, Cock JH, Gomez KA (1976). Laboratory Manual for Physiological Studies of Rice. 3 rd edn. Los Banos, Laguna: International Rice Research Institute. pp. 17-22.
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