Chin Bull Bot ›› 2016, Vol. 51 ›› Issue (5): 679-683.doi: 10.11983/CBB15181

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An Optimized Method to Determine Silicon Content in Rice

Yuwei Jia1†, Ruilin Yang2†, Yang Zhang1, Juanjuan Fang1, Hui Chen1*   

  1. 1College of Life Sciences, Shanxi Normal University, Linfen 041004, China
    2Center of Analysis and Test, Shanxi Normal University, Linfen 041004, China
  • Received:2015-10-10 Accepted:2016-02-14 Online:2016-09-27 Published:2016-09-01
  • Contact: Jia Yuwei,Yang Ruilin,Chen Hui
  • About author:

    # Co-first authors


To establish an easier and more accurate method to determine silicon content in rice, several main parameters of silicon molybdenum-blue spectrophotometry were optimized in combination with 2 sample pretreatments, digestion and autoclaving. By using ascorbic acid as a reductant, with the color development time of the silicon molybdenum yellow and the stable time of silicon molybdenum blue for 5 and 25 min, respectively, optimal silicon content in rice was achieved at 600 nm wavelength by molybdenum-blue spectrophotometry. For sample pretreatment, the digestion method was more effective for silicon extraction than autoclaving. Briefly, the optimized method facilitates the determination of silicon content in rice and other plants and could lay a theoretical foundation for further research of the mechanism of silicon absorbance and transportation.

Figure 1

Optimization of conditions of silicon molybdenum blue spectrophotometric and comparison between two methods of samples pretreatment for determination of silicon content (A) The full wavelength scan figure of three reducing agents (A+B liquid, ascorbic acid, and ammonium iron (II) sulfate); (B) Stable time of silicon molybdenum blue (means±SD, n=9); (C) Silicon standard curve; (D) Comparison of the silicon content in rice samples by using digestion and autoclaving method (means±SD, n=9). Different capital letters represent significant difference across the same samples at different methods (P<0.05, n=9); Different lowercase letters represent significant difference across the same methods at different samples (P<0.05, n=9)."

Table 1

Comparison of measurement using absorbance at the 600 and 812 nm wavelength"

Wavelength (nm) Observation number Minimum OD value Maximum OD value OD value (means±SD)
600 9 0.540 0.557 0.5468±0.006686
812 9 1.621 1.646 1.6302±0.011904

Table 2

Analysis of color development time of silicon molybdenum yellow"

Group Observation number Minimum OD value Maximum OD value OD value (means±SD)
Boiling water bath 30 s 9 0.529 0.532 0.5308±0.0011 c
Standing 5 min 9 0.534 0.536 0.5348±0.0007 a
Standing 10 min 9 0.534 0.536 0.5348±0.0008 a
Standing 15 min 9 0.532 0.535 0.5333±0.0013 ab
Standing 20 min 9 0.528 0.535 0.5321±0.0030 b
Standing 30 min 9 0.528 0.535 0.5320±0.0031 b
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