Chin Bull Bot ›› 2016, Vol. 51 ›› Issue (5): 631-638.doi: 10.11983/CBB15096

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Chlorophyll Fluorescence Response Characteristics of Typical Emergent Plants Under Different Total Nitrogen Gradient

Zhaoning Gong1,2,3,4*, Yunbao Fan1,2,3,4, Hui Liu1,2,3,4, Wenji Zhao1,2,3,4   

  1. 1College of Resource Environment and Tourism, Capital Normal University, Beijing 100048, China
    2Key Laboratory of 3D Information Acquisition and Application of Ministry of Education, Beijing 100048, China
    3Key Laboratory of Resources Environment and GIS of Beijing Municipal, Beijing 100048, China
    4Base of the State Laboratory of Urban Environmental Processes and Digital Modeling, Beijing 100048, China
  • Received:2015-06-01 Accepted:2016-04-26 Online:2016-09-27 Published:2016-09-01
  • Contact: Gong Zhaoning E-mail:gongzhn@163.com
  • About author:

    # Co-first authors

Abstract:

Measurements and analysis of chlorophyll fluorescence can reflect changes in plant leaf photochemical efficiency and have been increasingly used in plant ecological monitoring. This study used the Mencheng Lake wetland park, supplied with reclaimed water, as a study area, and Phragmites australis, Typha angustifolia and Zizania latifolia as typical emergent plants. We studied the chlorophyll fluorescence response characteristics of plants under different total nitrogen gradient by measuring leaf-scale chlorophyll fluorescence parameters outdoors and the corresponding water total nitrogen content indoors. Minimal fluorescence (Fo) and maximal fluorescence (Fm) were increased and nitrogen content in water was elevated. Maximum quantum efficiency of photochemistry in photosystem II in the dark (Fv/Fm) and quantum efficiency of PSll (ΦPSll) affected by nitrogen increased at first and then plateaued when nitrogen content reached the 15 to 20 mg·L-1 interval. Photochemical quenching (qP) tended to decrease after the first increase, but nonphotochemical quenching (NPQ) changed without obvious regularity. When water total nitrogen content was at the 15-20 mg·L-1 level, photochemical reaction was abated and photosynthesis was inhibited. Also, fluorescence parameters of different types of emergent plants were not the same; photosynthesis was significantly stronger in the growth stage (June) than the mature stage (September).

Figure 1

Location map and sampling sites of study area"

Table 1

Water quality status in Mencheng Lake wetland park"

Water quality parameters Minimal value Maximal value Mean value Standard deviation Class II Class V
pH 8.78 9.90 9.34 0.32 6-9 6-9
Dissolved oxygen (mg·L-1) 2.53 10.53 6.23 2.03 6 2
Total nitrogen (mg·L-1) 5.73 20.10 16.50 5.11 0.50 2.00

Figure 2

Responses of Fo and Fm in leaf of three plants to total nitrogen content in water Fo: Minimal fluorescence yield of the dark-adapted state; Fm: Maximal fluorescence yield of the dark-adapted state"

Table 2

Responses of Fv/Fm and ΦPSII in leaf of three plants to total nitrogen content in water"

Vegetation type Water total nitrogen
content (mg·L-1)
Number of
samples
June September
Fv/Fm ΦPSII Fv/Fm ΦPSII
Phragmites australis 5-10 20 0.49 0.31 0.46 0.27
10-15 24 0.60 0.40 0.55 0.37
15-20 36 0.58 0.43 0.57 0.41
Typha angustifolia 5-10 12 0.59 0.35 0.55 0.30
10-15 8 0.70 0.47 0.60 0.39
15-20 20 0.71 0.49 0.59 0.38
Zizania latifolia 5-10 6 0.48 0.36 0.34 0.34
10-15 12 0.59 0.33 0.54 0.36
15-20 14 0.60 0.25 0.56 0.32

Figure 3

Responses of qP and NPQ in leaf of three plants to total nitrogen content in water qP: Photochemical quenching; NPQ: Nonphotochemical quenching"

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