Chin Bull Bot ›› 2016, Vol. 51 ›› Issue (1): 16-23.doi: 10.11983/CBB15007

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Effect of Rhizosphere Ventilation on Growth of Cotton Seedlings Under Salt Stress

Lin Qi1, Xinfu Bai1*, Weihao Niu1, Zhenhua Zhang2   

  1. 1College of Life Sciences, Ludong University, Yantai 264025, China
    2College of Geography and Planning, Ludong University, Yantai 264025, China
  • Received:2015-01-12 Accepted:2015-03-30 Online:2016-02-01 Published:2016-01-01
  • Contact: Bai Xinfu E-mail:bxf64@163.com
  • About author:? These authors contributed equally to this paper

Abstract:

We examined the effect of rhizosphere ventilation and salt stress on the growth of cotton seedings cultivated in nutrient solution. The changes in plant height, root volume, shoot and root biomasses as well as ash content in cotton seedlings were examined to investigate the effect of rhizosphere ventilation on the growth of cotton under salt stress. Salt stress inhibited the growth of cotton and led to decreased plant height, leaf area and dry weight; poor ventilation also decreased plant height, dry weight and mineral element absorption. A further comparison of the effect of rhizosphere ventilation and the combined effect of rhizosphere ventilation with salt stress showed a prominent effect of salt stress on plant height and total biomass, with a striking effect of rhizosphere ventilation on root volume, root biomass, root-to-shoot ratio and mineral element absorption (i.e., the adverse effect of salt stress was mainly exhibited on stems and leaves, and that of rhizosphere ventilation on root growth). In addition, the variation in cotton seedlings in terms of plant height, root volume, leaf area, root biomass and total biomass in the rhizosphere ventilation conditions was smaller than those in seedlings without ventilation. Rhizosphere ventilation could increase plantlet height, leaf area, dry weight, thereby alleviating the adverse effect of salt stresses.

Key words: cotton, rhizosphere ventilation, salt stress, growth

Figure 1

Changes in O2 content in culture solutions of different treatmentsA: Aeration+0 mmol·L-1 NaCl; B: No aeration+0 mmol·L-1 NaCl; C: Aeration+100 mmol·L-1 NaCl; D: No aeration+100 mmol·L-1 NaCl; E: Aeration+200 mmol·L-1 NaCl; F: No aeration+200 mmol·L-1 NaCl"

Figure 2

Comparison of the increments in plant height (A) and root volume (B) in different treatmentsA-F see Figure 1. Significant differences (P<0.05) were indicated with different lowercase letters."

Table 1

Analysis of the variance of the physiological indices in cotton seedlings under salt stress and ventilation"

Sources of variation Plant
height
Root
volume
Single
leaf area
Specific
leaf area
Total
biomass
Root
biomass
Root-shoot
ratio
Ash
content
Salt concention F value 174.56 43.42 91.43 2.56 106.84 61.75 24.40 89.03
P 0.000 0.000 0.000 0.943 0.000 0.000 0.000 0.000
Ventilation F value 16.63 360.63 416.34 16.33 67.76 236.02 349.23 724.15
P 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Interaction F value 11.47 22.15 2.99 5.73 4.48 9.02 13.69 10.50
P 0.000 0.000 0.065 0.008 0.020 0.001 0.000 0.000

Table 2

The salt stress resulted variance of the indices in cotton seedlings under different ventilation"


Parameters
Aeration No aeration
Maximum
value
Minimum
value
Coefficient of
variability
Maximum
value
Minimum
value
Coefficient of
variability
Plant height (cm) 16.40±2.09 8.56±0.89 31.57 17.06±1.20 3.84±0.67 62.66
Root volume (cm3·plantlet-1) 11.40±1.45 7.98±2.05 17.54 6.60±0.87 1.18±0.15 79.39
Single leaf area (cm2) 137.78±6.18 109.39±10.22 11.52 101.73±6.26 60.93±5.46 24.90
Specific leaf area (cm2·g-1) 342.82±20.83 332.69±24.60 1.56 337.02±24.02 279.85±16.62 9.95
Total biomass (g·plantlet-1) 4.91±0.35 3.28±0.24 15.14 4.47±0.34 2.28±0.18 22.13
Root biomass (g·plantlet-1) 1.11±0.11 0.85±0.04 11.25 0.86±0.05 0.46±0.04 21.94
Root-shoot ratio 0.35±0.01 0.29±0.02 7.07 0.25±0.01 0.23±0.01 2.85
Ash content (%) 13.18±0.27 11.41±0.14 7.23 10.55±0.24 9.69±0.24 4.31

Figure 3

Comparison of the increments in single leaf area (A) and specific leaf area (B) in different treatmentsA-F see Figure 1. Significant differences (P<0.05) were indicated with different lowercase letters."

Figure 4

Comparison of shoot/root/total biomass (A) and root-shoot ratio (B) in different treatments A-F see Figure 1. Significant differences (P<0.05) were indicated with different lowercase letters."

Figure 5

Comparison of the increments in ash content in different treatments A-F see Figure 1. Significant differences (P<0.05) were indicated with different lowercase letters."

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