植物学报 ›› 2015, Vol. 50 ›› Issue (3): 394-404.DOI: 10.3724/SP.J.1259.2015.00394

• 专题论坛 • 上一篇    

pH敏感型荧光蛋白及其在植物细胞生物学中的应用

薛轶群1, 宋凯1, 范路生1, 万迎朗2, 林金星1,2,*()   

  1. 1中国科学院植物研究所, 北京 100093
    2北京林业大学生物科学与技术学院, 北京 100083
  • 收稿日期:2014-06-04 接受日期:2014-09-09 出版日期:2015-05-01 发布日期:2015-04-08
  • 通讯作者: 林金星
  • 作者简介:

    ? 共同第一作者

  • 基金资助:
    国家重点基础研究发展规划(No.2011CB809103)、国家自然科学基金创新研究群体(No.31121065)、北京林业大学杰出青年人才培育计划(No.JC2013-2)、新世纪优秀人才计划(No.NECT-12-0785)和国家自然科学基金(No.31271433)

pH-sensitive Fluorescent Proteins and Their Applications in Plant Cell Biology

Yiqun Xue1, Kai Song1, Lusheng Fan1, Yinglang Wan2, Jinxing Lin1, 2, *   

  1. 1Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
    2College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
  • Received:2014-06-04 Accepted:2014-09-09 Online:2015-05-01 Published:2015-04-08
  • Contact: Lin Jinxing
  • About author:

    ? These authors contributed equally to this paper

摘要:

pH敏感型荧光蛋白, 即pHluorin, 是荧光强度及光谱特征随环境pH值的变化而改变的一类荧光蛋白。人们通过对密码子使用偏好和特定剪切位点的修饰, 已使pHluorin及其衍生物成功地在动物、植物和真菌细胞中正常表达, 为测量细胞内微环境pH值的变化, 并研究活细胞内依赖或导致pH变化的生理过程提供了有力工具。该文总结了目前已报道的pH敏感型荧光蛋白的种类及特性, 并对其在细胞生物学, 特别是植物细胞生物学中的应用进行了详细介绍。随着报告基因技术及检测方法的不断改进, pHluorin将在植物科学领域发挥更大的作用。

Abstract:

pHluorin is a pH-sensitive variant of green fluorescent protein; its fluorescence signals display a strong pH dependence. Since the codon usage was optimized and the site of aberrant splicing was removed, pHluorin and its derivatives have been successfully expressed and used in animal, plant and fungi cells. These pH sensors have helped shed light on measure of the difference in pH of cellular microenvironment and many cell functions for which intracellular pH is an important modulator. Here, we review the characteristics of known pHluorins and their applications in cell biology, particularly plant cell biology. With the improvements in pHluorins by genetic engineering and the advancing in detection technology, the future for pHluorins in plant science is exciting.