植物学报 ›› 2010, Vol. 45 ›› Issue (03): 337-344.doi: 10.3969/j.issn.1674-3466.2010.03.005

• 研究报告 • 上一篇    下一篇

NaHCO3胁迫下星星草根中Ca2+与Ca2+-ATPase 的超微细胞化学定位

范玲玲1; 陈刚3; 陈义芳3; 周卫东3; 戴绍军1*; 孙国荣2*

  

  1. 1东北林业大学生命科学学院, 林木遗传育种与生物技术教育部重点实验室, 哈尔滨 1500402滨州职业学院, 滨州 256603; 3扬州大学生物科学与技术学院测试中心, 扬州 225009
  • 收稿日期:2009-06-16 修回日期:2009-11-18 出版日期:2010-03-01 发布日期:2010-05-01
  • 通讯作者: 戴绍军; 孙国荣

Ultracytochemical Localization of Ca2+ and Ca2+-ATPase in the Root of Puccinellia tenuiflora Under NaHCO3 Stress

Lingling Fan1; Gang Chen3; Yifang Chen3; Weidong Zhou3; Shaojun Dai1*; Guorong Sun2*   

  1. 1Northeast Forestry University, Harbin 150040, China;
    2Binzhou Polytechnic College, Binzhou 256603, China;
    3Yangzhou University, Yangzhou 225009, China
  • Received:2009-06-16 Revised:2009-11-18 Online:2010-03-01 Published:2010-05-01
  • Contact: Shaojun Dai;Guorong Sun

摘要: 利用焦锑酸盐和磷酸铅沉淀技术分别对NaHCO3胁迫条件下星星草(Puccinellia tenuiflora)根中Ca2+和Ca2+-ATPase 进行超微细胞化学定位研究, 旨在进一步探讨Ca2+在NaHCO3胁迫诱导胞内信号转导过程中的作用, 以及Ca2+-ATPase活性定位变化与NaHCO3胁迫下星星草抗盐碱能力的关系。结果表明: 在正常状态下, 根毛区细胞质内Ca2+较少, 主要位于质膜附近和液泡中, Ca2+-ATPase主要定位于质膜和液泡膜, 有一定活性。在0.448%NaHCO3胁迫下, 根毛区细胞质中Ca2+增多, 液泡中Ca2+减少, 且主要集中于液泡膜附近, 质膜和液泡膜Ca2+-ATPase活性明显升高。在1.054%NaHCO3胁迫下,细胞质中分布的Ca2+增多, 而液泡中Ca2+极少, Ca2+-ATPase活性也降低。以上结果表明, Ca2+亚细胞定位和Ca2+-ATPase活性变化在星星草响应NaHCO3胁迫的信号传递过程中具有重要作用。

Abstract: We aimed to further investigate the relationships between the localization of calcium and Ca2+-ATPase and the resistance capacity of Puccinellia tenuiflora against saline-alkali stress. We analyzed ultracytochemical localization of calcium and Ca2+-ATPase in the root of P. tenuiflora under NaHCO3 stress by potassium pyroantimonate precipitation and a lead-phosphate precipitation technique, respectively. In the normal condition, calcium antimonate precipitation deposits were low in cytoplasm and mainly concentrated within the plasma membrane and vacuoles in the root hair zone. Ca2+-ATPase had a certain activity and mainly localized in the plasma membrane and tonoplasts. Moreover, under 0.448% NaHCO3 stress, the distribution of Ca2+ precipitation particles increased in the cytoplasm, decreased in the vacuole, and mainly concentrated near tonoplasts. Meanwhile, the activity of Ca2+-ATPase was significantly increased in the plasma membrane and tonoplasts. Furthermore, under 1.054% NaHCO3, Ca2+ precipitation particles continued to increase in the cytoplasm but were absent in the vacuole. The activity of Ca2+-ATPase was also reduced. All these results highlight that the changes in Ca2+ localization and Ca2+-ATPase activity play important roles in P. tenuiflora resistance to saline-alkali stress.

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