植物学报

• 研究论文 •    下一篇

耐寒睡莲花瓣中花青素苷组成及其与花色的关系

朱满兰1,2, 王亮生2*, 张会金2, 徐彦军3, 郑绪辰1,2, 王丽金2,4   

  1. 1南京农业大学园艺学院, 南京 210095;
    2中国科学院植物研究所北京植物园/资源植物研发重点实验室, 北京 100093
    3中国农业大学理学院, 北京 100094;
    4中国科学院研究生院, 北京 100049
  • 收稿日期:2012-03-30 修回日期:2012-05-17 出版日期:2012-09-01 发布日期:2012-07-24
  • 通讯作者: 王亮生 E-mail:wanglsh@ibcas.ac.cn

Relationship Between the Composition of Anthocyanins and Flower Color Variation in Hardy Water Lily (Nymphaea spp.) Cultivars

Manlan Zhu1,2, Liangsheng Wang2*, Huijin Zhang2, Yanjun Xu3, Xuchen Zheng1,2, Lijin Wang2,4   

  1. 1College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;

    2Beijing Botanical Garden/Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;

    3Department of Applied Chemistry, China Agricultural University, Beijing 100094, China;

    4Graduate University of the Chinese Academy of Sciences, Beijing 100049, China
  • Received:2012-03-30 Revised:2012-05-17 Online:2012-09-01 Published:2012-07-24
  • Contact: Liangsheng Wang E-mail:wanglsh@ibcas.ac.cn

摘要: 睡莲(Nymphaea spp.)为多年生水生观赏花卉。以耐寒睡莲不同花色的119个栽培品种为材料, 利用高效液相色谱(HPLC-DAD)和液质联用技术(HPLC-ESI-MSn)测定了其花瓣中的花青素苷成分。采用英国皇家园艺学会比色卡(RHSCC)和国际照明委员会(CIE)制定的CIEL*a*b*表色系统测量了57个品种的花色, 运用多元线性回归方法分析花色与花青素苷组成之间的关系。结果表明: 耐寒睡莲花瓣中含有14种花青素苷, 其中飞燕草素-3-半乳糖-5-乙酰-半乳糖苷(Dp3Ga5acetylGa)、飞燕草素-3-鼠李糖-(1→2)-半乳糖苷(Dp3Rh(1→2)Ga)、矢车菊素-3-半乳糖-(1→2)-半乳糖苷(Cy3Ga(1→2)Ga)、矢车菊素-3-乙酰-半乳糖-(1→2)-半乳糖苷(Cy3acetylGa(1→2)Ga)、矢车菊素-3-没食子酰-半乳糖苷(Cy3galloylGa)、飞燕草素-3-乙酰-葡萄糖苷(Dp3acetylG)、飞燕草素-3-葡萄糖苷(Dp3G)和矢车菊素-3-半乳糖-半乳糖-半乳糖苷(Cy3GaGaGa)8个组分在耐寒睡莲中为首次报道。Dp3Ga、Dp3galloylGa、Cy3Ga(1→2)Ga和Cy3galloylGa是决定耐寒睡莲呈色的关键花青素苷。

Abstract: Water lily (Nymphaea spp.) is a perennial aquatic flower plant. We investigated color of 57 hardy water lily cultivars by the Royal Horticultural Society Colour Chart (RHSCC) and International Commission on Illumination CIEL*a*b* scale. High-performance liquid chromatography (HPLC) with photodiode array detection (DAD) and HPLC electrospray ionization mass spectrometry (HPLC-ESI-MSn) identified 14 anthocyanins. Eight compounds were identified in the petals of 119 hardy water lily cultivars for the first time. Multiple linear regression revealed that Dp3Ga, Dp3galloylGa, Cy3Ga(1→2)Ga and Cy3galloylGa compounds were key pigments in petal color. We discuss differences in anthocyanin components between hardy and tropical water lily and blue color breeding of hardy water lily.

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