星蕨体外快繁技术

葛晓青, 李梦瑶, 黄衡宇, 张爱丽

Rapid Propagation Technology of Microsorum punctatum in Vitro

Xiaoqing Ge, Mengyao Li, Hengyu Huang, Aili Zhang

图4 对星蕨孢子体绿色球状小体的诱导及绿色球状小体(GGB)植株的再生与驯化 (A)以孢子体为材料诱导GGBs (bar=1 cm); (B) 孢子体长出绿色球状小体(bar=1 mm); (C) 根状茎分离出绿色球状小体(bar=1 mm); (D), (E) 绿色球体培育成的GGBs聚合体((D) bar=1 mm; (E) (bar=1 cm); (F) GGBs聚合体中分离出单个GGB (bar=500 μm); (G), (H) GGBs分化长出幼孢子体((G) bar=5 mm; (H) bar=1 mm); (I) 孢子体根状茎长出GGB (bar=1 cm); (J) GGB增殖(bar=1 cm); (K) GGB分化成苗(bar=1 cm); (L) 培养60天后孢子体生根(bar=1 cm); (M)-(P) 孢子体驯化((M) bar=5 cm; (N)-(P) bars=3 cm)。因孢子体驯化生长空间有限(M), 后续将其转入面积较大的隔间驯化(N)-(P)。

Figure 4 Green globular induction of sporophytes and regeneration and domestication of green globular body (GGB) plants of Microsorum punctatum (A) GGBs induced by sporophyte (bar=1 cm); (B) Spore grows green globular bodies (bar=1 mm); (C) Rhizomes isolated green globules (bar=1 mm); (D), (E) GGBs polymer cultivated from e-green spheroids ((D) bar=1 mm; (E) bar=1 cm); (F) A single GGB was isolated from GGBs polymer (bar=500 μm); (G), (H) GGBs differentiated into young sporophytes ((G) bar=5 mm; (H) bar=1 mm); (I) Sporophyte rhizomes grow GGB (bar=1 cm); (J) GGB multiplication (bar=1 cm); (K) GGB differentiate into plantlets (bar=1 cm); (L) The sporophyte took root after 60 days of culture (bar=1 cm); (M)-(P) Sporophytic domestication ((M) bar=5 cm; (N)-(P) bars=3 cm). Due to limited space for the domestication and growth of spores (M), they were later transferred to larger compartments for domestication (N)-(P).