星蕨体外快繁技术

葛晓青, 李梦瑶, 黄衡宇, 张爱丽

Rapid Propagation Technology of Microsorum punctatum in Vitro

Xiaoqing Ge, Mengyao Li, Hengyu Huang, Aili Zhang

图2 原叶体增殖和幼孢子体诱导 (A) 转接15天后的原叶体团(bar=2 cm); (B) 转接30天后的原叶体团(bar=2 cm); (C) 转接45天后的原叶体团(bar=2 cm); (D) 转接60天后的原叶体团(bar=2 cm); (E) 原叶体(bar=500 μm); (F) 精子器(bar=40 μm); (G) 局部颈卵器(bar=200 μm); (H) 颈卵器(bar=40 μm); (I) 加水培养20天后的原叶体和幼孢子体(bar=2 cm); (J) 加水培养60天后的原叶体和幼孢子体(bar=2 cm); (K) 加水培养90天后的原叶体和幼孢子体(bar=2 cm); (L) 孢子体生根培养(bar=2 cm)

Figure 2 Proliferation of prothallus and induction of young sporophytes (A) Prolobular mass was transferred after 15 days (bar=2 cm); (B) Prolobular mass was transferred after 30 days (bar=2 cm); (C) Prolobular mass was transferred after 45 days (bar=2 cm); (D) Prolobular mass was transferred after 60 days (bar=2 cm); (E) Gametophyte (bar=500 μm); (F) Antheridium (bar=40 μm); (G) Local archegonium (bar=200 μm); (H) Archegonium (bar=40 μm); (I) Prothallus and young sporophyte cultured with water after 20 days (bar=2 cm); (J) Prothallus and young sporophyte cultured with water after 60 days (bar=2 cm); (K) Prothallus and young sporophyte cultured with water after 90 days (bar=2 cm); (L) Sporophyte rooting culture (bar=2 cm)