菊花品种万代风光再生及遗传转化体系的建立

李晶晶, 李艳飞, 王安琪, 王佳颖, 邓成燕, 卢敏, 马剑英, 戴思兰

Establishment of Regeneration and Genetic Transformation System for Chrysanthemum × morifolium ‘Wandai Fengguang’

Jingjing Li, Yanfei Li, Anqi Wang, Jiaying Wang, Chengyan Deng, Min Lu, Jianying Ma, Silan Dai

图1 植物表达载体pBI121的结构 RB: T-DNA区段右边界; NOS-pro: 农杆菌胭脂碱合成酶基因启动子; KanR: 卡那霉素抗性筛选标记基因; NOS-ter: 农杆菌胭脂碱合成酶基因终止子; CaMV35S-pro: 花椰菜花叶病毒35S启动子; CcVIT: 矢车菊液泡铁离子转运蛋白基因; LB: T-DNA区段左边界

Figure 1 The structure of plant expression vector pBI121 RB: Right border of T-DNA region; NOS-pro: Agrobacterium tumefaciens nopaline synthase gene promoter; KanR: Kanamycin resistance screening marker gene; NOS-ter: A. tumefaciens nopaline synthase gene terminator; CaMV35S-pro: Cauliflower mosaic virus 35S promoter; CcVIT: Centaurea cyanus vacuolar iron transport protein gene; LB: Left border of T-DNA region