一个新的黄瓜叶色突变体鉴定、初定位及转录组分析

赵蔓雅, 孙倩楠, 徐晶晶, 段恬妮, 蔡锦涛, 周婧, 范婷婷, 萧浪涛, 王若仲

Identification, Mapping and Transcriptome Analysis of a New Leaf Color Mutant in Cucumber

Manya Zhao, Qiannan Sun, Jingjing Xu, Tianni Duan, Jintao Cai, Jing Zhou, Tingting Fan, Langtao Xiao, Ruozhong Wang

图6 ycl与XYYH-3-1间的RNA-Seq分析 (A) 差异表达基因火山图; (B) 差异表达基因KEGG途径富集散点图; (C) GO term二级分类统计结果; (D) 转录组结果的qRT-PCR验证。BP: 生物过程; MF: 分子功能; CC: 细胞组分; WT: 野生型; DEG: 差异表达基因; FPKM: 每千碱基转录本每百万映射片段数; FC: 倍性变化。**表示差异极显著(t检验, P<0.01)。

Figure 6 RNA-Seq analysis of differential expression between ycl and XYYH-3-1 (A) Differentially expressed genes volcano map; (B) Scatter plot of differentially expressed genes KEGG pathway enrichment; (C) GO term secondary classification statistics; (D) The qRT-PCR verification of transcriptome results. BP: Biological process; MF: Molecular function; CC: Cellular component; WT: Wild type; DEG: Differential expressed gene; FPKM: Fragments per kilobase per million mapped reads; FC: Fold Change. ** denote extremely significant differences (t-test, P<0.01).