发根农杆菌介导的野葛毛状根遗传转化体系

曾文丹, 严华兵, 吴正丹, 尚小红, 曹升, 陆柳英, 肖亮, 施平丽, 程冬, 龙紫媛, 李婕宇

Agrobacterium rhizogenes-mediated Transformation System of Pueraria lobata Hairy Roots

Zeng Wendan, Yan Huabing, Wu Zhengdan, Shang Xiaohong, Cao Sheng, Lu Liuying, Xiao Liang, Shi Pingli, Cheng Dong, Long Ziyuan, Li Jieyu

图4 野葛转基因毛状根GFP荧光观察及PCR检测 (A) 毛状根GFP荧光观察(bar=1 cm); (B) PCR检测(M: DL2000 DNA marker; N: 超纯水; V: K599; P: 阳性对照; 1-20: 不同转基因毛状根株系)

Figure 4 Green fluorescence assay and PCR analysis of the GFP in Pueraria lobata transgenic hairy roots (A) GFP green fluorescence assay of hairy roots (bar=1 cm); (B) PCR detection (M: DL2000 DNA marker; N: ddH2O; V: K599; P: Positive control; 1-20: Different transgenic hairy root lines)