毛建草愈伤组织诱导及植株再生

田旭平, 岳康杰, 王佳丽, 刘慧欣, 史子尹, 亢红伟

Callus Induction and Plant Regeneration of Dracocephalum rupestre

Xuping Tian, Kangjie Yue, Jiali Wang, Huixin Liu, Ziyin Shi, Hongwei Kang

表7 6-BA、TDZ和IAA组合对愈伤组织分化的影响

Table 7 Effects of combination 6-BA, TDZ and IAA on callus differentiation

Type No. 6-BA (mg∙L-1) TDZ (mg∙L-1) IAA (mg∙L-1) Adventitious buds differentiation rate (%) Adventitious roots differentiation rate (%) Callus from leaves of field planted indivi- dual F1 1.0 0.5 0.05 - 8.97±2.34 c F2 1.0 1.0 1.0 - 17.83±5.09 ab F3 1.0 2.0 0.5 14.39±3.23 b - F4 2.0 0.5 1.0 - 23.44±9.12 a F5 2.0 1.0 0.5 19.07±2.96 b 7.15±2.17 c F6 2.0 2.0 0.05 17.43±5.99 b 2.31±1.97 c F7 3.0 0.5 0.5 66.37±13.14 a 2.69±0.81 c F8 3.0 1.0 0.05 23.29±8.06 b - F9 3.0 2.0 1.0 7.94±2.01 c 28.91±13.04 a Callus from leaves of tissue-cultured seedling F1 1.0 0.5 0.05 - 14.32±4.23 ab F2 1.0 1.0 1.0 - 23.64±9.34 a F3 1.0 2.0 0.5 22.35±9.33 b 4.52±1.96 c F4 2.0 0.5 1.0 - 17.61±3.22 ab F5 2.0 1.0 0.5 1.32±0.42 bc 9.02±2.83 c F6 2.0 2.0 0.05 53.48±9.89 a 6.49±2.02 c F7 3.0 0.5 0.5 9.46±2.31 bc 19.03±7.93 a F8 3.0 1.0 0.05 19.83±6.99 b 12.87±4.85 ab F9 3.0 2.0 1.0 9.10±2.41 bc 8.99±3.31 c