毛建草愈伤组织诱导及植株再生

田旭平, 岳康杰, 王佳丽, 刘慧欣, 史子尹, 亢红伟

Callus Induction and Plant Regeneration of Dracocephalum rupestre

Xuping Tian, Kangjie Yue, Jiali Wang, Huixin Liu, Ziyin Shi, Hongwei Kang

表6 6-BA、TDZ和NAA组合对愈伤组织分化的影响

Table 6 Effects of combination of 6-BA, TDZ and NAA on callus differentiation

Type No. 6-BA (mg∙L-1) TDZ (mg∙L-1) NAA (mg∙L-1) Adventitious buds differentiation rate (%) Adventitious roots differentiation rate (%) Callus from leaves of field planted indivi- dual F1 1.0 0.5 0.05 2.13±0.19 d 3.21±0.13 d F2 1.0 1.0 1.0 - 31.69±7.11 a F3 1.0 2.0 0.5 8.92±2.83 c 9.51±2.24 c F4 2.0 0.5 1.0 6.43±1.71 cd 23.49±11.31 b F5 2.0 1.0 0.5 11.21±3.02 c 6.91±2.17 cd F6 2.0 2.0 0.05 19.46±6.33 ab - F7 3.0 0.5 0.5 5.64±1.91 d 2.02±0.17 d F8 3.0 1.0 0.05 21.29±9.12 a - F9 3.0 2.0 1.0 15.82±3.64 b 4.09±1.22 d Callus from leaves of tissue-cultured seedling F1 1.0 0.5 0.05 - 21.23±6.71 b F2 1.0 1.0 1.0 - 29.88±9.45 a F3 1.0 2.0 0.5 - 19.01±6.54 bc F4 2.0 0.5 1.0 - 12.56±4.32 cd F5 2.0 1.0 0.5 - 14.67±3.03 c F6 2.0 2.0 0.05 - 8.03±2.31 d F7 3.0 0.5 0.5 - 11.34±2.12 d F8 3.0 1.0 0.05 - 3.34±1.42 de F9 3.0 2.0 1.0 - 3.49±1.92 de