毛建草愈伤组织诱导及植株再生
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田旭平, 岳康杰, 王佳丽, 刘慧欣, 史子尹, 亢红伟
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Callus Induction and Plant Regeneration of Dracocephalum rupestre
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Xuping Tian, Kangjie Yue, Jiali Wang, Huixin Liu, Ziyin Shi, Hongwei Kang
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表5 6-BA与TDZ组合对愈伤组织分化的影响
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Table 5 Effects of combination 6-BA with TDZ on callus differentiation
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| Type | No. | 6-BA (mg∙L-1) | TDZ (mg∙L-1) | Adventitious buds
differentiation rate (%) | Adventitious roots
differentiation rate (%) | | Callus from leaves of field planted individual | F1 | 0 | 0.5 | - | 12.43±3.26 ab | | F2 | 0 | 1.0 | - | 7.41±2.12 c | | F3 | 0 | 2.0 | - | 3.94±1.12 d | | F4 | 1.0 | 0 | - | 15.61±4.36 a | | F5 | 1.0 | 0.5 | 3.07±1.04 b | - | | F6 | 1.0 | 1.0 | - | 3.41±1.27 d | | F7 | 1.0 | 2.0 | - | 12.37±3.75 ab | | F8 | 2.0 | 0 | - | 10.91±3.12 b | | F9 | 2.0 | 0.5 | 3.95±0.92 b | - | | F10 | 2.0 | 1.0 | 4.53±0.87 b | 8.76±2.21 bc | | F11 | 2.0 | 2.0 | - | 7.55±1.32 c | | F12 | 3.0 | 0 | 4.66±1.41 b | 6.38±2.63 c | | F13 | 3.0 | 0.5 | 16.71±5.22 a | - | | F14 | 3.0 | 1.0 | - | 4.21±1.21 c | | F15 | 3.0 | 2.0 | 4.39±1.21 b | - | | Callus from leaves of tissue-cultured seedling | F1 | 0 | 0.5 | - | - | | F2 | 0 | 1.0 | - | 4.52±0.95 bc | | F3 | 0 | 2.0 | - | 4.74±1.39 c | | F4 | 1.0 | 0 | - | 4.58±1.51 bc | | F5 | 1.0 | 0.5 | - | 1.92±0.82 c | | F6 | 1.0 | 1.0 | - | 9.17±2.42 a | | F7 | 1.0 | 2.0 | - | 6.11±2.21 b | | F8 | 2.0 | 0 | - | 3.43±1.02 c | | F9 | 2.0 | 0.5 | 3.44±1.32 b | 8.32±2.31 a | | F10 | 2.0 | 1.0 | - | 6.41±2.13 b | | F11 | 2.0 | 2.0 | 2.91±0.71 bc | 3.59±1.33 c | | F12 | 3.0 | 0 | - | 2.33±0.93 c | | F13 | 3.0 | 0.5 | 2.37±0.69 bc | 7.15±1.12 ab | | F14 | 3.0 | 1.0 | 3.17±1.21 b | - | | F15 | 3.0 | 2.0 | 7.66±2.12 a | - |
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