毛建草愈伤组织诱导及植株再生

田旭平, 岳康杰, 王佳丽, 刘慧欣, 史子尹, 亢红伟

Callus Induction and Plant Regeneration of Dracocephalum rupestre

Xuping Tian, Kangjie Yue, Jiali Wang, Huixin Liu, Ziyin Shi, Hongwei Kang

表4 6-BA与2,4-D和IAA组合对叶片愈伤组织诱导的影响

Table 4 Effects of combination 6-BA with 2,4-D and IAA on the callus induction of leaves

Type 6-BA (mg∙L-1) 2,4-D (mg∙L-1) IAA (mg∙L-1) Occurrence time (d) Induction rate (%) Callus growth state Leaves of field planted indivi- dual 0.5 0.1 0.1 9.17±3.67 ab 60.33±13.05 b Yellow-green, loose, soft texture 0.5 0.5 1.0 12.32±3.63 bc 44.87±10.33 c Brown, loose, soft texture 0.5 1.0 0.5 12.58±2.42 bc 41.24±11.13 c Brown, loose, soft texture 1.0 0.1 1.0 10.16±2.21 b 84.51±14.22 a Yellow-green, loose, softer texture 1.0 0.5 0.5 11.00±2.52 b 61.39±12.65 b Yellow, loose, soft texture 1.0 1.0 0.1 7.69±2.44 a 77.59±14.25 a Yellow, loose, softer texture 2.0 0.1 0.5 7.42±1.09 a 79.08±17.24 a Yellow, resembling water stains, soft texture 2.0 0.5 0.1 8.38±3.87 ab 71.22±12.13 ab Yellow with water stain, soft texture 2.0 1.0 1.0 11.33±2.21 b 63.49±13.41 b Pale yellow, loose, soft texture Leaves of tissue-cultured seedling 0.5 0.1 0.1 13.08±2.42 bc 47.62±9.43 c Green, compact, hard texture 0.5 0.5 1.0 14.41±4.38 bc 53.41±13.22 bc Green, compact, hard texture 0.5 1.0 0.5 16.11±6.38 c 55.76±10.16 bc Green, compact, somewhat hard texture 1.0 0.1 1.0 9.33±2.46 ab 42.57±9.25 c Yellow-green, loose, softer texture 1.0 0.5 0.5 11.67±3.50 b 50.91±13.22 c Pale yellow, loose, softer texture 1.0 1.0 0.1 13.67±2.50 bc 44.59±11.17 c Pale yellow, loose, softer texture 2.0 0.1 0.5 8.58±2.12 a 86.73±11.29 a Yellow-green, loose, softer texture 2.0 0.5 0.1 7.32±3.31 a 70.23±17.05 b Yellow, loose, soft texture 2.0 1.0 1.0 11.33±2.22 b 67.15±13.21 b Green, compact, hard texture