毛建草愈伤组织诱导及植株再生

田旭平, 岳康杰, 王佳丽, 刘慧欣, 史子尹, 亢红伟

Callus Induction and Plant Regeneration of Dracocephalum rupestre

Xuping Tian, Kangjie Yue, Jiali Wang, Huixin Liu, Ziyin Shi, Hongwei Kang

表3 6-BA与2,4-D组合对叶片愈伤组织诱导的影响

Table 3 Effects of combination 6-BA with 2,4-D on the callus induction of leaves

Type 6-BA (mg∙L-1) 2,4-D (mg∙L-1) Occurrence time (d) Induction rate (%) Callus growth state Leaves of field planted individual 0.5 0 10.25±2.46 b 40.38±8.73 b Yellow, compact, hard texture 0.5 0.1 11.42±1.79 bc 25.77±6.33 c Pale yellow, loose, soft texture 0.5 0.5 10.67±2.65 b 41.83±6.86 bc Brownish-yellow, compact, somewhat hard texture 0.5 1.0 15.00±4.66 c 44.08±9.91 bc Brownish-yellow, resembling water stains, soft texture 1.0 0 7.58±2.29 a 65.38±13.16 a Pale yellow, loose, softer texture 1.0 0.1 10.08±3.20 b 43.33±12.02 b Pale yellow, dense, softer texture 1.0 0.5 12.92±3.71 bc 51.16±15.02 ab Pale yellow, loose, softer texture 1.0 1.0 12.00±2.76 bc 56.32±9.31 ab Yellow, compact, somewhat hard texture 2.0 0 6.92±2.17 a 67.52±13.25 a Pale yellow, loose, resembling water stains, soft texture 2.0 0.1 7.91±2.22 a 60.83±11.02 a Yellow, resembling water stains, soft texture 2.0 0.5 10.33±1.45 b 57.12±13.37 ab Yellow, compact, softer texture 2.0 1.0 13.67±2.05 bc 48.53±8.04 b Pale yellow, loose, softer texture Leaves of tissue-cultured seedling 0.5 0 11.08±3.69 b 47.51±11.05 b Yellow, compact, hard texture 0.5 0.1 16.42±3.08 c 44.17±9.31 b Pale yellow, loose, soft texture 0.5 0.5 11.08±2.44 b 39.98±8.74 bc Pale yellow, loose, hard texture 0.5 1.0 16.33±5.30 c 19.03±6.33 c Yellow, loose, soft texture 1.0 0 10.33±3.48 ab 55.62±10.15 ab Yellow-green, loose, softer texture 1.0 0.1 15.17±3.17 c 49.71±10.63 b Green loose, softer texture 1.0 0.5 10.42±3.44 ab 44.54±9.63 b Yellow, compact, hard texture 1.0 1.0 10.92±2.51 ab 38.05±8.94 bc Pale yellow, loose, softer texture 2.0 0 8.17±2.27 a 68.23±14.06 a Yellow-green, loose, softer texture 2.0 0.1 11.67±2.54 b 66.11±11.82 a Green, loose, softer texture 2.0 0.5 9.42±3.33 a 53.42±11.44 ab Pale yellow, loose, softer texture 2.0 1.0 9.83±3.44 a 50.76±14.13 b Pale yellow, loose, softer texture