毛建草愈伤组织诱导及植株再生

田旭平, 岳康杰, 王佳丽, 刘慧欣, 史子尹, 亢红伟

Callus Induction and Plant Regeneration of Dracocephalum rupestre

Xuping Tian, Kangjie Yue, Jiali Wang, Huixin Liu, Ziyin Shi, Hongwei Kang

表2 6-BA与IAA组合对叶片愈伤组织诱导的影响

Table 2 Effects of combination 6-BA with IAA on the callus induction of leaves

Type 6-BA (mg∙L-1) IAA (mg∙L-1) Occurrence time (d) Induction rate (%) Callus growth state Leaves of field planted individual 0.5 0 10.25±4.60 b 40.37±6.89 b Yellow, compact, and with a hard texture 0.5 0.1 15.25±3.72 c 39.09±8.97 bc Green, loose, with a softer texture 0.5 0.5 9.58±1.40 ab 33.28±7.29 bc Pale yellow, loose, with a harder texture 0.5 1.0 8.08±1.96 a 26.72±5.74 c Yellow, resembling water stains, soft texture 1.0 0 7.58±2.29 a 65.33±6.56 a Pale yellow, loose, softer texture 1.0 0.1 14.67±3.08 c 55.46±5.64 ab Green, compact, harder texture 1.0 0.5 10.83±3.33 b 47.51±6.74 b Pale yellow, loose, soft texture 1.0 1.0 9.58±2.39 ab 33.72±9.32 bc Green loose, soft texture 2.0 0 6.92±2.17 a 67.55±6.54 a Pale yellow, loose, watery appearance, soft texture 2.0 0.1 11.67±1.20 b 63.29±7.96 a Pale green, loose, soft texture 2.0 0.5 12.16±2.46 bc 54.29±8.57 ab Yellow-green, loose, softer texture 2.0 1.0 14.00±3.43 c 41.31±9.03 b Green, loose, softer texture Leaves of tissue- cultured seedling 0.5 0 11.08±2.69 b 47.81±5.75 b Yellow, compact, hard texture 0.5 0.1 12.00±3.38 bc 34.52±6.34 bc Yellow-green, loose, soft texture 0.5 0.5 13.00±3.43 bc 31.96±4.75 bc Yellow-green, compact, softer texture 0.5 1.0 18.08±4.17 c 24.07±7.22 c Yellow-green, loose, soft texture 1.0 0 10.33±2.48 b 55.67±4.21 ab Yellow-green, loose, softer texture 1.0 0.1 10.33±2.58 b 51.34±1.99 ab Yellow-green, loose, soft texture 1.0 0.5 11.33±3.42 b 46.21±11.23 b Yellow-green, loose, softer texture 1.0 1.0 15.42±3.22 c 39.14±9.41 bc Yellow-green, loose, soft texture 2.0 0 8.17±1.27 a 68.73±13.47 a Yellow-green, loose, softer texture 2.0 0.1 9.67±2.30 ab 55.24±6.91 ab Yellow-green, loose, soft texture 2.0 0.5 9.88±2.65 ab 51.33±13.54 ab Green, loose, softer texture 2.0 1.0 10.33±2.79 b 46.61±14.26 b Yellow-green, loose, softer texture